Estudos para o desenvolvimento de novo metodo de avaliação de qualidade proteica utilizando larvas de Chrysomya chloropyga (Wiedmann)
Tania Teresinha de Souza Leal
DISSERTAÇÃO
Português
(Broch.)
T/UNICAMP L473e
[Studies for the development of new methodology for evaluating quality protein using larvae of Chrysomya chloropyga (WIEDMANN)]
Campinas, SP : [s.n.], 1980.
128f. : il.
Orientador : Aluisio Jose Antunes
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos e Agricola
Resumo: As larvas das espécies Chrysomya albiceps, Chrysomya chloropyga e Chrysomya megacephala foram inicialmente utilizadas neste estudo. A espécie C. chloropyga, foi selecionada por seu comportamento semelhante em dieta natural (carcaça) e na dieta de leite e levedura (STOFFOLANO, 1974)....
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Resumo: As larvas das espécies Chrysomya albiceps, Chrysomya chloropyga e Chrysomya megacephala foram inicialmente utilizadas neste estudo. A espécie C. chloropyga, foi selecionada por seu comportamento semelhante em dieta natural (carcaça) e na dieta de leite e levedura (STOFFOLANO, 1974). Verificou-se inicialmente, o efeito de diferentes niveis de proteína na dieta, no desenvolvimento das larvas, utilizando-se a:rcD fonte proteica leite em pó integral, leite em pó integral combinado com levedura, caseína e caseína combinada com levedura, nos níveis de 2, 4, 6, 8 e 10%. Nas dietas de leite e de leite e levedura, as porcentagens de ganho de peso foram proporcionais ao nível de proteína das dietas nos níveis 2 e 4%. Nos níveis 6, 8 e 10%, não houve crescimento, provavelmente devido à alta concentração de sólidos nas dietas. Com a utilização de caseína e caseína combinada com levedura, obtivemos respostas de crescimento proporcionais ao níve1 de proteína das dietas até o nível 8% para as dietas de caseína e levedura e até o nível 10%, para as dietas de caseína. Como as dietas contendo levedura apresentaram resultados superiores aos obtidos somente com leite ou caseína como fontes proteicas e, sendo levedura uma fonte rica em ácido ribonucleico, este substituiu a levedura nas dietas de caseína, obtendo-se resultados superiores aos até então obtidos.- A série de dietas contendo caseína e RNA foi por este motivo utilizada como padrão nos ensaios subseqüentes. Verificamos então, respostas das larvas quando proteinas de diferentes origens eram adicionadas às dietas. No primeiro grupo de dietas as farinhas das leguminosas feijão e soja foram utilizadas, testando-se também o efeito de tratamento térmico e o de suplementação com metionina no desenvolvimento das larvas. A suplementação de feijão crú com metionina não aumentou a porcentagem de ganho de peso das larvas, o que ocorreu após o tratamento térmico e também com a suplementação da farinha tratada. Nas dietas com farinha de soja crua, quando suplementadas com metionina, as larvas apresentaram porcentagem de ganho de peso maior do que as dietas não suplementadas. O tratamento térmico também melhorou a qualidade das dietas. Os cereais arroz, milho e trigo foram utilizados como contendo cereal como única fonte proteica, para milho e trigo. A suplementação das dietas com aminoácidos limitantes produziu resultados superiores ou próximos aos obtidos com as dietas complementadas. Um último ensaio foi realizado utilizando-se ovo integral liofilizado como fonte proteica, obtendo-se resultados bastante satisfatórios, embora as dietas não contivessem RNA adicionado, confirmando que o desempenho da dieta depende do balanço adequado de nutrientes
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Abstract: The growth of Chrysomya albiceps, Chrysomya chloropyga and Chrysomya megaceplala larvae toward a natural (mice carcass) and milk-yeast diets was studied. The larva of C. chloropyga
was selected because of its identical growth in both diets. Experiments were carried out to study the effect... Ver mais Abstract: The growth of Chrysomya albiceps, Chrysomya chloropyga and Chrysomya megaceplala larvae toward a natural (mice carcass) and milk-yeast diets was studied. The larva of C. chloropyga
was selected because of its identical growth in both diets. Experiments were carried out to study the effect of different protein levels and different protein sources as well. Four pratein sources were utilized in this study: l)dried whole milk, 2) dried whole milk-plusyeast, 3) casein and 4) casein
cornbined with yeast. The protein levels were 2, 4, 6, 8 and 10% for each one of the diets. In the dried whole milk and dried whole milk-plus-yeast the gain in body weight was proportional to the protein content of the diets, at the levels af 2 and 4%. At 6, 8 and 10% the growth was impaired, probably due to the high solid concentration in the diets. When the caseio and the casein-plus-yeast diets were utilized, the growth was proportional to the protein content of the diets up to the leveI of 8%, in the case of the casein plus-yeast diet, and up to 10% for the casein diet. When yeast was incorporated to the diets, the growth rates were always higher than those obtained without it. This result was not surprising sinee yeasts are a good natural source of ribonucleic acid. The addition of RNA to the casein diet showed even better results, in terms of growth, than the casein-plus-yeast diet. For this reason, the casein diet to which RNA was added, was taken as the standard diet throughout this study. The effect af different protein saurces on the growth rates of C. chloropyga larvas was investigated in another set of experiments. The ability of bean (Phaseolus vulgaris) and soy-bean (Glycine max) proteins to support grawth as well as the effect of heat treatment and L-methionine supplementation were studied. The larvae did not respond to the supplementation of the unheated bean protein with L-methionine, however the growth rates were higher when the flour was heat-treated and when the heated-treated flour was supplemented with L-methionine. In the case of unheated soy flour, there was a better growth response after L-methionine addition; heated soy flour supported higher growth rates than the unheat flour both with and without L-methionine addition. Rice corn and wheat were also testes as protein sources for the C. Chloropyga larvae. Corn and wheat when supplemented with limiting amino acids elicited a better growth response than the unsupplemented sources. Supplementation of corn, rice and wheat with bean and soy-bean protein was beneficial to the growth of C. Chloropyga larvae. Dried whole egg vlas also tested, giving higher growth rates when compared with any other protein source Ver menos
was selected because of its identical growth in both diets. Experiments were carried out to study the effect... Ver mais Abstract: The growth of Chrysomya albiceps, Chrysomya chloropyga and Chrysomya megaceplala larvae toward a natural (mice carcass) and milk-yeast diets was studied. The larva of C. chloropyga
was selected because of its identical growth in both diets. Experiments were carried out to study the effect of different protein levels and different protein sources as well. Four pratein sources were utilized in this study: l)dried whole milk, 2) dried whole milk-plusyeast, 3) casein and 4) casein
cornbined with yeast. The protein levels were 2, 4, 6, 8 and 10% for each one of the diets. In the dried whole milk and dried whole milk-plus-yeast the gain in body weight was proportional to the protein content of the diets, at the levels af 2 and 4%. At 6, 8 and 10% the growth was impaired, probably due to the high solid concentration in the diets. When the caseio and the casein-plus-yeast diets were utilized, the growth was proportional to the protein content of the diets up to the leveI of 8%, in the case of the casein plus-yeast diet, and up to 10% for the casein diet. When yeast was incorporated to the diets, the growth rates were always higher than those obtained without it. This result was not surprising sinee yeasts are a good natural source of ribonucleic acid. The addition of RNA to the casein diet showed even better results, in terms of growth, than the casein-plus-yeast diet. For this reason, the casein diet to which RNA was added, was taken as the standard diet throughout this study. The effect af different protein saurces on the growth rates of C. chloropyga larvas was investigated in another set of experiments. The ability of bean (Phaseolus vulgaris) and soy-bean (Glycine max) proteins to support grawth as well as the effect of heat treatment and L-methionine supplementation were studied. The larvae did not respond to the supplementation of the unheated bean protein with L-methionine, however the growth rates were higher when the flour was heat-treated and when the heated-treated flour was supplemented with L-methionine. In the case of unheated soy flour, there was a better growth response after L-methionine addition; heated soy flour supported higher growth rates than the unheat flour both with and without L-methionine addition. Rice corn and wheat were also testes as protein sources for the C. Chloropyga larvae. Corn and wheat when supplemented with limiting amino acids elicited a better growth response than the unsupplemented sources. Supplementation of corn, rice and wheat with bean and soy-bean protein was beneficial to the growth of C. Chloropyga larvae. Dried whole egg vlas also tested, giving higher growth rates when compared with any other protein source Ver menos
Estudos para o desenvolvimento de novo metodo de avaliação de qualidade proteica utilizando larvas de Chrysomya chloropyga (Wiedmann)
Tania Teresinha de Souza Leal
Estudos para o desenvolvimento de novo metodo de avaliação de qualidade proteica utilizando larvas de Chrysomya chloropyga (Wiedmann)
Tania Teresinha de Souza Leal
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