Resumo: O Coriandrum sativum L. conhecido popularmente como coentro é citado como antidiabético, antiinflamatório, hipoglicemiante e antibacteriano. O gênero Candida faz parte da microbiota da cavidade oral e sob circunstâncias especiais produzem candidíase e infecções sistêmicas. O objetivo desse trabalho foi avaliar a atividade do óleo essencial (OE), extratos hexânico (EHE) e hidroalcoólico (EHA) de folhas de C. sativum frente as diferentes espécies de Candida e identificar seus principais constituintes. Para obtenção dos EHE e EHA foram utilizadas 10g das folhas secas e moídas, originando-se 0,43 g do EHE e 2,34 g do EHA, com rendimentos de 4,3% e 24,3%, respectivamente. O OE (2,06 g - rendimento 0,03%) foi obtido por hidrodestilação em sistema do tipo Clevenger a partir de 7500 g de folhas frescas, sendo fracionado em coluna seca e originando sete frações (FOE) com diferentes polaridades, as quais foram analisadas por CCD e CG-EM. Os constituintes majoritários identificados do OE foram álcoois e aldeídos de cadeia linear: 1- decanol (24,17%), 2E-decenol (18,05%) e 2Z-dodecenol (17,55%). Os OE, EHE e EHA foram testados frente as diferentes espécies de Candida: C. albicans CBS 562; C.krusei CBS 573; C. parapsilosis CBS 604; C. dubliniensis CBS 7987 e C. tropicalis CBS 94. As amostras do OE e extratos foram solubilizadas em água destilada e tween 1% para concentração em solução de 4 mg/mL, testando-se concentrações de 1000 Yg/mL a 15,6Yg/mL. O OE apresentou forte inibição frente as diferentes espécies de Candida com MICs de 125 a 500 Yg/mL, enquanto os EHE e EHA inibiram apenas C. parapsilosis com MIC de 250 Yg/mL. As frações 4, 5 e 6 do OE enriquecidas em álcoois apresentaram forte inibição e amplo espectro antimicrobiano, com MICs de 7 a 250 Yg/mL, evidenciando que esses compostos são responsáveis pela atividade antimicrobiana frente as diferentes espécies de Candida , enquanto as frações FOE 1 a 3 ricas em aldeídos, com MICs de 31 a 1000 Yg/mL, apresentaram atividade antimicrobiana moderada. Esses resultados demonstram o potencial uso de Coriandrum sativum no combate a leveduras do gênero Candida Ver menos

Abstract: Coriandrum sativum L. (Umbelliferae / Apiaceae), known as coriander, is a popular cooking condiment in Brazil; it has been reported as an anti-inflammatory, anti-diabetic and anti-bacterial agent. Different Candida spp., which might lead to complications such as skin candidosis or even systemic infections, are commonly found in the oral microbiota. The aim of this study was to evaluate the activity of the essential oil (EO) and hexanic and hydroalcoholic extracts of C. sativum against different species of Candida, as well as to identify their main constituents. To obtain the hexanic (HEE) and hydroalcoholic extracts (HAE), 10 g of dried leaves of C. sativum was used to attain 0.43 g of HEE and 2.34 g of HAE, with yields of 4.3% and 24.3%, respectively. Its essential oil (2.06 g ¿ yield 0.03 %) was obtained by hydrodistillation using the Clevenger system where 7500 g of its fresh leaves and dry-column chromatography was used to fraction the oil into seven fractions with different polarities. These fractions were then analyzed by TLC and GC-MS. The main constituents identified in the essential oil were long-chain alcohols: 1-decanol (24.17 %); 2E-decenol (18.05 %); and 2Z-dodecenol (17,55 %) and aldehydes. The HEE, HAE, and EO were tested against C. albicans CBS 562, C. krusei CBS 573, C. parapsilosis CBS 604, C. dubliniensis CBS 7987, and C. Tropicalis CBS 94. Samples of EO, HEE and HAE were dissolved in distilled water with 1% tween to obtain a solution concentration of 4 mg/mL and 1000 µg/mL to 15.6 µg/mL of which was tested against Candida spp. The EO showed antibacterial activity against most species of Candida, with MICs ranging from 125 µg/mL to 500 µg/mL, while the extracts inhibited only C. parapsilosis with a MIC of 250 µg/mL. Alcohol-enriched fractions 4, 5 and 6 of EO showed strong inhibition and broad antimicrobial spectrum, with MICs ranging from 7 to 250 µg/mL, suggesting that alcohol might be responsible for the antimicrobial activity against the various species of Candida tested. While the fractions 1 to 3 rich in aldehydes, with MICs ranging from 31 to 1000 µg/mL, showed moderate antimicrobial activity against the species of Candida tested. Results showed potential antimicrobial activity of Coriandrum sativum against Candida spp (yeast infections) Ver menos