Analise microbiologica de canais radiculares e radiografica de dentes de cães com lesão periapical induzida
Helena Rosa Campos Rabang
DISSERTAÇÃO
Português
(Broch.)
T/UNICAMP R112a
Campinas, SP : [s.n.], 2003.
153 p.
Orientador: Francisco Jose de Souza Filho
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Resumo: O valor do cão na pesquisa em Endodontia é reconhecido pela freqüência com que é utilizado. Várias investigações são conduzidas visando observar alterações e reparo na região periapical deste modelo. Sendo os microrganismos os principais agentes etiológicos das alterações pulpo-periapicais,...
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Resumo: O valor do cão na pesquisa em Endodontia é reconhecido pela freqüência com que é utilizado. Várias investigações são conduzidas visando observar alterações e reparo na região periapical deste modelo. Sendo os microrganismos os principais agentes etiológicos das alterações pulpo-periapicais, o conhecimento da microbiota presente em canais radiculares de cães se faz importante. O objetivo deste trabalho foi analisar microbiologicamente canais radiculares, e radiograficamente lesões periapicais induzidas em dentes de cães por um método. De um total de 5 animais, foram utilizados 26 pré-molares inferiores. Após os acessos coronários as polpas foram removidas e os dentes permaneceram expostos ao meio oral por um período de 120 dias. Controles clínico-radiográficos foram procedidos e as imagens das reações periapicais induzidas foram avaliadas pelo software Imagelab 2.4, mensurando área e perímetro. Coletas das amostras dos canais radiculares foram realizadas após 120 dias. Foram utilizados meios de transporte, cultura e incubação que propiciam o crescimento de bactérias anaeróbias estritas. Foram isoladas 70 bactérias cultiváveis, que pertenciam a 34 diferentes espécies, variando de O a 9 por canal. Anaeróbios facultativos corresponderam a 77,14%, anaeróbios estritos a 22,8% do total de bactérias isoladas, com uma microbiota predominantemente Gram-positiva. Os gêneros bacterianos mais freqüentemente isolados nos canais foram: Streptococcus, Staphy/ococcus, Neisseria, Propionibacterium, Actinomyces e Prevotella. As médias das áreas das imagens radiográficas das lesões foram de 0,06 cm2, para os segundos pré-molares, 0,12 cm2para os terceiros pré-molares e 0,17 cm2 para os quartos pré-molares. Concluímos que a microbiota de canais radiculares de dentes de cães com lesão periapical induzida, por exposição ao meio oral, após
120 dias, é predominantemente anaeróbia facultativa e Gram-positiva, representada pelos gêneros Streptococcus e Staphy/ococcus. Os resultados sugerem existência de relação positiva entre comprimento de dente e tamanho de
lesão periapical Ver menos
120 dias, é predominantemente anaeróbia facultativa e Gram-positiva, representada pelos gêneros Streptococcus e Staphy/ococcus. Os resultados sugerem existência de relação positiva entre comprimento de dente e tamanho de
lesão periapical Ver menos
Abstract: The value of dogs in endodontic research is well known by the regularity of their use. Many investigations are conducted in order to observe periapical response and repair on the periapical tissues of this experimental model.
Microorganisms are the main etiological agents in pulpal and... Ver mais Abstract: The value of dogs in endodontic research is well known by the regularity of their use. Many investigations are conducted in order to observe periapical response and repair on the periapical tissues of this experimental model.
Microorganisms are the main etiological agents in pulpal and periapical disorders, therefore comprehension of the dog root canal microflora is important. The objective of this study was to analyse microbiological canal roots and radiograph
induced periapical lesions in dog's teeth. Twenty six lower premolars of five mongrel dogs were used. Their chambers were accessed and their pulps removed and left open to the oral environment for the total period of 120 days. Clínical and
radiographic controls were used. The radiographs were evaluated by Imagelab 2.4 software to measure the area and perimeter images of the induced periapical lesions. Samples from the root canais were collected after 120 days. Methodology
for handling, culture and incubation for growth of strict anaerobe species was used. A total of 70 cultivable isolates were recovered from 34 different species, rallging from 0to 9 per canal. Facultative anaerobe species comprised 77.14% of the
samples and 22.8% were strict anaerobic species with one microbiota predominantly Gram-positive. The most frequent genera recovered from the canais were: Streptococcus, Staphy/ococcus, Neisseria, Propionibacterium, Actinomyces and Prevotella. Measurements of the radiographic image data of the lesions were 0.06 cm2 for the second premolars, 0.12 cm2for the third premolars and 0.17 cm2 for the fourth premolars. In conclusion, the root canal microbiota of dog teeth with induced periapical lesion, by exposure to the oral environment, is predominantly composed by facultative anaerobes and Gram-positive bacteria, represented by Streptococcus and Staphy/ococcus genera. The results suggest a positive relationship between the tooth length and size of the periapical lesion Ver menos
Microorganisms are the main etiological agents in pulpal and... Ver mais Abstract: The value of dogs in endodontic research is well known by the regularity of their use. Many investigations are conducted in order to observe periapical response and repair on the periapical tissues of this experimental model.
Microorganisms are the main etiological agents in pulpal and periapical disorders, therefore comprehension of the dog root canal microflora is important. The objective of this study was to analyse microbiological canal roots and radiograph
induced periapical lesions in dog's teeth. Twenty six lower premolars of five mongrel dogs were used. Their chambers were accessed and their pulps removed and left open to the oral environment for the total period of 120 days. Clínical and
radiographic controls were used. The radiographs were evaluated by Imagelab 2.4 software to measure the area and perimeter images of the induced periapical lesions. Samples from the root canais were collected after 120 days. Methodology
for handling, culture and incubation for growth of strict anaerobe species was used. A total of 70 cultivable isolates were recovered from 34 different species, rallging from 0to 9 per canal. Facultative anaerobe species comprised 77.14% of the
samples and 22.8% were strict anaerobic species with one microbiota predominantly Gram-positive. The most frequent genera recovered from the canais were: Streptococcus, Staphy/ococcus, Neisseria, Propionibacterium, Actinomyces and Prevotella. Measurements of the radiographic image data of the lesions were 0.06 cm2 for the second premolars, 0.12 cm2for the third premolars and 0.17 cm2 for the fourth premolars. In conclusion, the root canal microbiota of dog teeth with induced periapical lesion, by exposure to the oral environment, is predominantly composed by facultative anaerobes and Gram-positive bacteria, represented by Streptococcus and Staphy/ococcus genera. The results suggest a positive relationship between the tooth length and size of the periapical lesion Ver menos
Souza Filho, Francisco José de, 1950-2014
Orientador
Uzeda, Milton de
Avaliador
Analise microbiologica de canais radiculares e radiografica de dentes de cães com lesão periapical induzida
Helena Rosa Campos Rabang
Analise microbiologica de canais radiculares e radiografica de dentes de cães com lesão periapical induzida
Helena Rosa Campos Rabang
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