Tipagem molecular e perfil de suscetibilidade aos antifungicos de isolados ambientais e clinicos de Cryptococcus neoformans na cidade e região de Campinas, São Paulo
Ana Cecilia Nastrini Delgado
DISSERTAÇÃO
Português
(Broch.)
T/UNICAMP D378t
Campinas, SP : [s.n.], 2002.
153f. : il.
Orientadores: Maria Cecilia Barisson Villares, Maria Luiza Moretti Branchini
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
Resumo: A criptococose representa importante micose oportunista de ocorrência freqüente e grave nos pacientes atendidos no HC-UNICAMP, em especial nos pacientes com síndrome da imunodeficiência adquirida (Aids). O presente estudo foi proposto para aprimorar o conhecimento de C. neoformans isolados...
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Resumo: A criptococose representa importante micose oportunista de ocorrência freqüente e grave nos pacientes atendidos no HC-UNICAMP, em especial nos pacientes com síndrome da imunodeficiência adquirida (Aids). O presente estudo foi proposto para aprimorar o conhecimento de C. neoformans isolados de pacientes com criptococose e sua correlação com o meio ambiente. Foram estudadas 52 cepas clínicas e 55 cepas ambientais (excretas de pombos) de C. neoformans procedentes da cidade e região de Campinas, São Paulo, Brasil. As cepas de C. neoformans foram isoladas em meio ágar "cornmeal" com ácido cafeico e as variedades e sorotipos foram identificados, respectivamente, pelo meio de canavanina-glicina-azul de bromotimol (CGB) e pelo teste de aglutinação em lâmina utilizando o kit "Crypto Check latron Co. Japão". Foi realizado estudo do perfil genômico através da eletroforese em campo pulsátil (PFGE), utilizando o sistema "Counter-Clamped Homogeneus Electric Field" (CHEF). O estudo da suscetibilidade de C. neoformans frente a anfotericina B, 5-fluorocitosina, fluconazol e itraconazol foi realizado pelo método da microdiluição em caldo, segundo o National Committee for Clinical Laboratory Standards (NCCLS)...Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital
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Abstract: Cryptococcosis represents an important opportunistic mycosis with frequent and serious occurance in patients hospitalized at the Hospital and Clinics of UNICAMP, specially in immunocompromised patients. The present study was proposed to
perfect the knowledge of C. neoformans isolated from... Ver mais Abstract: Cryptococcosis represents an important opportunistic mycosis with frequent and serious occurance in patients hospitalized at the Hospital and Clinics of UNICAMP, specially in immunocompromised patients. The present study was proposed to
perfect the knowledge of C. neoformans isolated from patients with cryptococcosis and its correlation with the environment. Fifty-two clinical isolates and 55 environmental (pigeon droppings) isolates of C. neoformans were investigated, from Campinas, which is located in the state of São Paulo in Brazil. The clinical and environmental isolates were identified to species level based on micromorphological and biochemical characteristics and confirmed to be C. neoformans var. neoformans based on the reaction of canavanine-glycine-bromthymol blue agar. Serotyping was performed by slide agglutination test (Crypto Check; latron Co., Japan). Electrophoretic karyotype (EK) analysis was performed by Counter-Clamped Homogeneous Electric Field (CHEF DR-III). All the
isolates were tested by broth microdilution method, performed according to the NCCLS guidelines for amphotericin B (AMB), fluconazole (FLCZ), itraconazole (ITCZ), and fluorocytosine (5-FC)...Note: The complete abstract is available with the full electronic digital thesis or dissertations Ver menos
perfect the knowledge of C. neoformans isolated from... Ver mais Abstract: Cryptococcosis represents an important opportunistic mycosis with frequent and serious occurance in patients hospitalized at the Hospital and Clinics of UNICAMP, specially in immunocompromised patients. The present study was proposed to
perfect the knowledge of C. neoformans isolated from patients with cryptococcosis and its correlation with the environment. Fifty-two clinical isolates and 55 environmental (pigeon droppings) isolates of C. neoformans were investigated, from Campinas, which is located in the state of São Paulo in Brazil. The clinical and environmental isolates were identified to species level based on micromorphological and biochemical characteristics and confirmed to be C. neoformans var. neoformans based on the reaction of canavanine-glycine-bromthymol blue agar. Serotyping was performed by slide agglutination test (Crypto Check; latron Co., Japan). Electrophoretic karyotype (EK) analysis was performed by Counter-Clamped Homogeneous Electric Field (CHEF DR-III). All the
isolates were tested by broth microdilution method, performed according to the NCCLS guidelines for amphotericin B (AMB), fluconazole (FLCZ), itraconazole (ITCZ), and fluorocytosine (5-FC)...Note: The complete abstract is available with the full electronic digital thesis or dissertations Ver menos
Villares, Maria Cecilia Barisson, 1948-2016
Orientador
Branchini, Maria Luiza Moretti
Coorientador
Paula, Claudete Rodrigues
Avaliador
Tipagem molecular e perfil de suscetibilidade aos antifungicos de isolados ambientais e clinicos de Cryptococcus neoformans na cidade e região de Campinas, São Paulo
Ana Cecilia Nastrini Delgado
Tipagem molecular e perfil de suscetibilidade aos antifungicos de isolados ambientais e clinicos de Cryptococcus neoformans na cidade e região de Campinas, São Paulo
Ana Cecilia Nastrini Delgado
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