Estudo morfologico, morfometrico e estereologico da placenta e tecidos fetais, na transmissão transplacentaria de Trypanosoma Cruzi em camundongos
Ana Amelia Carraro Abrahão
TESE
Português
(Broch.)
T/UNICAMP Ab82e
Campinas, SP : [s.n.], 2000.
120 p. : il.
Orientador: Ruberval Armando Lopes
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Resumo: O presente trabalho teve como objetivo estudar as alterações morfológicas das placentas e dos fetos de camundongos Mus musculus, Swiss, infectados com a cepa RAL de Trypanosoma cruzi, durante a prenhez, através de métodos histopatológicos, morfométricos e estereológicos. Fêmeas prenhas,...
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Resumo: O presente trabalho teve como objetivo estudar as alterações morfológicas das placentas e dos fetos de camundongos Mus musculus, Swiss, infectados com a cepa RAL de Trypanosoma cruzi, durante a prenhez, através de métodos histopatológicos, morfométricos e estereológicos. Fêmeas prenhas, pesando aproximadamente 30-35 gramas, foram inoculadas i.p. com 2 x 105 tripomastigotas sangüícolas da cepa RAL de T.cruzi, no 70 dia da prenhez e sacrificadas por inalação com éter etílico no 190 dia da prenhez. Os animais do grupo controle foram inoculados com solução fisiológica e mantidos nas mesmas condições ambientais dos infectados. Foram determinados o peso e comprimento fetais, o peso e diâmetro placentários, bem como o comprimento do cordão umbilical dos animais pertencentes aos dois grupos experimentais. Além do exame histopatológico do material foram estudadas a cariometria e a estereologia das placentas e tecidos fetais (pâncreas, fígado e coração). Também estudou-se cariometricamente as células gigantes e os espongiotrofoblastos. Nos animais infectados, ocorreu uma queda no peso e diâmetro das placentas, bem como uma redução no tamanho e peso dos fetos, com diminuição do comprimento do cordão umbilical. Histopatologicamente, os animais infectados apresentaram parasitismo nas camadas decídua, esponjosa e labiríntica das placentas, com escassa reação inflamatória na decídua. As células gigantes apresentaram ninhos de amastigotas e mostraram pouca degeneração. Nos órgãos fetais estudados (pâncreas, fígado e coração) não foram encontrados ninhos de parasita. Os parâmetros nucleares das células gigantes mostraram-se significantemente diminuídos nos animais infectados quando comparados aos animais controles. O estudo estereológico da placenta mostrou que a região esponjosa apresentou volume relativo e volume absoluto menor no grupo infectado, sendo a diferença estatisticamente significante. Os volumes absolutos das regiões decídua e labiríntica das placentas dos animais infectados também apresentaram valores menores. O volume relativo, celular e citoplasmático das células gigantes das placentas dos animais infectados mostrou valores significantemente menores quando comparados com os animais do grupo controle. A densidade numérica e o número de células gigantes por placenta também foi significantemente menor no grupo infectado. Deste modo, as alterações funcionais provocadas por Trypanosoma cruzi sobre as células trofoblásticas gigantes e sobre os espongioblastos, podem atuar diretamente sobre o crescimento fetal, mediante uma perda de homeostasia da produção de lactogênio placentário, ou indiretamente mediante uma vascularização útero-placentária ineficiente, prejudicando desta maneira a nutrição fetaL
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Abstract: Morphologic alterations of placentas and foetus of Mus musculus infected with RAL strain of Trypanosoma cruzi were investigated by histopathological, morphometrical and stereological methods.
On the 7th day of pregnancy, ten female swiss mice, weighing approximately 30-35gr were i.p.... Ver mais Abstract: Morphologic alterations of placentas and foetus of Mus musculus infected with RAL strain of Trypanosoma cruzi were investigated by histopathological, morphometrical and stereological methods.
On the 7th day of pregnancy, ten female swiss mice, weighing approximately 30-35gr were i.p. inoculated with 2x105 blood trypomastigotes of RAL strain. The animals were sacrified with ethylic ether on the 19th day of pregnancy. Control group (non infected pregnant females) were i.p. inoculated with saline solution and kept in the same enviroment conditions of the infected counterparts. In both experimental groups, weight and lenght of foetus, diameter and weight of placenta as well as umbilical cord lenght were assessed. Histopathology, cariometry and stereology of placentas and fetal tis sues (liver, heart and pancreas) were performed. Cariometry of giant cells and spongyotrophoblasts were assessed too. Infected animals showed reduction of fetal weight and lenght, as well as the diameter and weight of placentas, with enshortened umbilical cord. Histopathologicaly, infected group displayed amastigote burdens in decidua, spongy and labyrinthic zone with scarce inflamatory reaction. Giant cells showed also amastigote burdens with few tissue disorganization. No parasite burdens were found in fetal organs (pancreas,liver and heart).Giant cells of infected animals showed significant reduction of nuclear parameters when compared to control group. The stereological study of placenta from infected animals revealed a spongy region with a smaller relative volume when compared to non infected counterparts although the absolute volume displayed significant differences in all regions of the placenta. The relative cellular and cytoplasmatic volume of giant cells as well as the number / mm3 and the total number / placenta, were significantly lower in infected group whencompared to non infected control animals. We conclude that, the functional alterations on spongioblasts and giant trophoblastic cells caused by RAL strain of T.cruzi can direct1y impair intrauterine fetal development through two distinct ways: directly by depressing production of placental lactogen or indirectly by leading to an inefficient uterus placental vascularization, impairing fetal nutrition Ver menos
On the 7th day of pregnancy, ten female swiss mice, weighing approximately 30-35gr were i.p.... Ver mais Abstract: Morphologic alterations of placentas and foetus of Mus musculus infected with RAL strain of Trypanosoma cruzi were investigated by histopathological, morphometrical and stereological methods.
On the 7th day of pregnancy, ten female swiss mice, weighing approximately 30-35gr were i.p. inoculated with 2x105 blood trypomastigotes of RAL strain. The animals were sacrified with ethylic ether on the 19th day of pregnancy. Control group (non infected pregnant females) were i.p. inoculated with saline solution and kept in the same enviroment conditions of the infected counterparts. In both experimental groups, weight and lenght of foetus, diameter and weight of placenta as well as umbilical cord lenght were assessed. Histopathology, cariometry and stereology of placentas and fetal tis sues (liver, heart and pancreas) were performed. Cariometry of giant cells and spongyotrophoblasts were assessed too. Infected animals showed reduction of fetal weight and lenght, as well as the diameter and weight of placentas, with enshortened umbilical cord. Histopathologicaly, infected group displayed amastigote burdens in decidua, spongy and labyrinthic zone with scarce inflamatory reaction. Giant cells showed also amastigote burdens with few tissue disorganization. No parasite burdens were found in fetal organs (pancreas,liver and heart).Giant cells of infected animals showed significant reduction of nuclear parameters when compared to control group. The stereological study of placenta from infected animals revealed a spongy region with a smaller relative volume when compared to non infected counterparts although the absolute volume displayed significant differences in all regions of the placenta. The relative cellular and cytoplasmatic volume of giant cells as well as the number / mm3 and the total number / placenta, were significantly lower in infected group whencompared to non infected control animals. We conclude that, the functional alterations on spongioblasts and giant trophoblastic cells caused by RAL strain of T.cruzi can direct1y impair intrauterine fetal development through two distinct ways: directly by depressing production of placental lactogen or indirectly by leading to an inefficient uterus placental vascularization, impairing fetal nutrition Ver menos
Lopes, Ruberval Armando
Orientador
Ribeiro, Rosa Domingues
Avaliador
Matteo, Miguel Angel Sala di
Avaliador
Kawazoe, Urara, 1946-
Avaliador
Ueta, Marlene Tiduko, 1941-
Avaliador
Estudo morfologico, morfometrico e estereologico da placenta e tecidos fetais, na transmissão transplacentaria de Trypanosoma Cruzi em camundongos
Ana Amelia Carraro Abrahão
Estudo morfologico, morfometrico e estereologico da placenta e tecidos fetais, na transmissão transplacentaria de Trypanosoma Cruzi em camundongos
Ana Amelia Carraro Abrahão
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