Agradecimentos: This work was financially supported by São Paulo Research Foundation (FAPESP) (#2014/02205-1, 2016/25112-4 and 2019/01678-7 [WASJ], 2018/21120-8; 2019/16904-2 [CA], 2014/21129-4 [DCM]), Brazilian National for Scientific and Technological Development (CNPq) process# MCTIC/CNPq 28/2018 406444/2018-8 - CA and FAEPEX-PRP/Unicamp (#519.292 - DCM). LBR, RLAL and JVF received CAPES-DS Master scholarships. C.G. received a CNPq scholarship (#142280/2017-8). We thank Dr. Fernanda R. Gadelha for her technical support and Ana J. Vincoletto for the assistance with promastigotes' morphology analysis Ver menos

Abstract: Leishmania amazonensis and L. braziliensis are the main etiological agents of the American Tegumentary Leishmaniasis (ATL). Taking into account the limited effectiveness and high toxicity of the current drug arsenal to treat ATL, novel options are urgently needed. Inspired by the fact that gold-based compounds are promising candidates for antileishmanial drugs, we studied the biological action of a systematic series of six (1)-(6) symmetric Au(I) benzyl and aryl-N-heterocyclic carbenes. All compounds were active at low micromolar concentrations with 50% effective concentrations ranging from 1.57 to 8.30 µM against Leishmania promastigotes. The mesityl derivative (3) proved to be the best candidate from this series, with a selectivity index ~13 against both species. The results suggest an effect of the steric and electronic parameters of the N-substituent in the activity. Intracellular infections were drastically reduced after 24h of (2)-(5) incubation in terms of infection rate and amastigote burden. Further investigations showed that our compounds induced significant parasites' morphological alterations and membrane permeability. Also, (3) and (6) were able to reduce the residual activity of three Leishmania recombinant cysteine proteases, known as possible targets for Au(I) complexes. Our promising results open the possibility of exploring gold complexes as leishmanicidal molecules to be further screened in in vivo models of infection Ver menos