Increased mTOR signaling and impaired autophagic flux are hallmarks of SARS-CoV-2 infection
Érika Pereira Zambalde, Thomaz Luscher Dias, Grazielle Celeste Maktura, Mariene R. Amorim, Bianca Brenha, Luana Nunes Santos, Lucas Buscaratti, João Gabriel de Angeli Elston, Mariana Camargo Silva Mancini, Isadora Carolina Betim Pavan, Daniel A. Toledo-Teixeira, Karina Bispo-dos-Santos, Pierina L....
Érika Pereira Zambalde, Thomaz Luscher Dias, Grazielle Celeste Maktura, Mariene R. Amorim, Bianca Brenha, Luana Nunes Santos, Lucas Buscaratti, João Gabriel de Angeli Elston, Mariana Camargo Silva Mancini, Isadora Carolina Betim Pavan, Daniel A. Toledo-Teixeira, Karina Bispo-dos-Santos, Pierina L. Parise, Ana Paula Morelli, Luiz Guilherme Salvino da Silva, Ícaro Maia Santos de Castro, Tatiana D. Saccon, Marcelo A. Mori, Fabiana Granja, Helder I. Nakaya, Jose Luiz Proenca-Modena, Henrique Marques-Souza, Fernando Moreira Simabuco
ARTIGO
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Agradecimentos: The authors acknowledge the National Institute of Science and Technology of Photonics Applied to Cell Biology (INFABIC) and the technical support of Mariana Ozello Baratti for her aid with confocal microscopy and Elzira Saviani and Thais Theizen for their technical support. Hernandes...
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Agradecimentos: The authors acknowledge the National Institute of Science and Technology of Photonics Applied to Cell Biology (INFABIC) and the technical support of Mariana Ozello Baratti for her aid with confocal microscopy and Elzira Saviani and Thais Theizen for their technical support. Hernandes F. Carvalho and Luis Lamberti Pinto da Silva for providing important primary and secondary antibodies related to markers of cellular and biological processes, and Guilherme Barbosa, Raissa G. Ludwig, and Thiago L. Knittel for thorough scientific discussions and critical reading of the manuscript. This work was supported by grants from FAEPEX-UNICAMP (2005/20; 2319/20; 2432/20; 2274/20), São Paulo Research Foundation (FAPESP) (2018/14933-2; 2018/14818-9; 2020/05284-0; 2014/50938-8; 2020/05346-6; 2020/04919-2; 2020/04558-0) and National Council for Scientific and Technological Development (CNPq) (465699/2014-6)
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Abstract: The COVID-19 (Coronavirus Disease 2019), caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), severely affects mainly individuals with pre-existing comorbidities. Here our aim was to correlate the mTOR (mammalian/mechanistic Target of Rapamycin) and autophagy...
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Abstract: The COVID-19 (Coronavirus Disease 2019), caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), severely affects mainly individuals with pre-existing comorbidities. Here our aim was to correlate the mTOR (mammalian/mechanistic Target of Rapamycin) and autophagy pathways with the disease severity. Through western blotting and RNA analysis, we found increased mTOR signaling and suppression of genes related to autophagy, lysosome, and vesicle fusion in Vero E6 cells infected with SARS-CoV-2 as well as in transcriptomic data mining of bronchoalveolar epithelial cells from severe COVID-19 patients. Immunofluorescence co-localization assays also indicated that SARS-CoV-2 colocalizes within autophagosomes but not with a lysosomal marker. Our findings indicate that SARS-CoV-2 can benefit from compromised autophagic flux and inhibited exocytosis in individuals with chronic hyperactivation of mTOR signaling
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2014/50938-8; 2018/14933-2; 2018/14818-9; 2020/05284-0; 2020/04919-2; 2020/04558-0
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
465699/2014-6
Aberto
Zambalde, Érika Pereira
Autor
Granja, Fabiana, 1979-
Autor
DOI: https://doi.org/10.3390/cimb45010023
Texto completo: https://www.mdpi.com/1467-3045/45/1/23
Increased mTOR signaling and impaired autophagic flux are hallmarks of SARS-CoV-2 infection
Érika Pereira Zambalde, Thomaz Luscher Dias, Grazielle Celeste Maktura, Mariene R. Amorim, Bianca Brenha, Luana Nunes Santos, Lucas Buscaratti, João Gabriel de Angeli Elston, Mariana Camargo Silva Mancini, Isadora Carolina Betim Pavan, Daniel A. Toledo-Teixeira, Karina Bispo-dos-Santos, Pierina L....
Érika Pereira Zambalde, Thomaz Luscher Dias, Grazielle Celeste Maktura, Mariene R. Amorim, Bianca Brenha, Luana Nunes Santos, Lucas Buscaratti, João Gabriel de Angeli Elston, Mariana Camargo Silva Mancini, Isadora Carolina Betim Pavan, Daniel A. Toledo-Teixeira, Karina Bispo-dos-Santos, Pierina L. Parise, Ana Paula Morelli, Luiz Guilherme Salvino da Silva, Ícaro Maia Santos de Castro, Tatiana D. Saccon, Marcelo A. Mori, Fabiana Granja, Helder I. Nakaya, Jose Luiz Proenca-Modena, Henrique Marques-Souza, Fernando Moreira Simabuco
Increased mTOR signaling and impaired autophagic flux are hallmarks of SARS-CoV-2 infection
Érika Pereira Zambalde, Thomaz Luscher Dias, Grazielle Celeste Maktura, Mariene R. Amorim, Bianca Brenha, Luana Nunes Santos, Lucas Buscaratti, João Gabriel de Angeli Elston, Mariana Camargo Silva Mancini, Isadora Carolina Betim Pavan, Daniel A. Toledo-Teixeira, Karina Bispo-dos-Santos, Pierina L....
Érika Pereira Zambalde, Thomaz Luscher Dias, Grazielle Celeste Maktura, Mariene R. Amorim, Bianca Brenha, Luana Nunes Santos, Lucas Buscaratti, João Gabriel de Angeli Elston, Mariana Camargo Silva Mancini, Isadora Carolina Betim Pavan, Daniel A. Toledo-Teixeira, Karina Bispo-dos-Santos, Pierina L. Parise, Ana Paula Morelli, Luiz Guilherme Salvino da Silva, Ícaro Maia Santos de Castro, Tatiana D. Saccon, Marcelo A. Mori, Fabiana Granja, Helder I. Nakaya, Jose Luiz Proenca-Modena, Henrique Marques-Souza, Fernando Moreira Simabuco
Fontes
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Current issues in molecular biology v. 45, n. 1, p. 327-336, 2023 |