Agradecimentos: Funding
The authors would like to thank the Coordination for the Improve ment of Higher Education Personnel (CAPES) for their support in con ducting this study – Financing Code 001. The authors acknowledge the Sao ˜ Paulo Research Foundation (FAPESP) for the financial support
(grant #2022/03268-3)
CRediT authorship contribution statement
Augusto Cesar ' Costa-Santos: Writing – review & editing, Writing – original draft, Validation, Methodology, Investigation, Formal analysis, Conceptualization. Ana Paula Rebellato: Writing – review & editing, Methodology, Investigation, Formal analysis. Gisele Marcondes Luz:
Writing – review & editing, Methodology, Formal analysis. Juliana Azevedo Lima Pallone: Writing – review & editing, Visualization, Su pervision, Resources, Project administration.
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper Ver menos

Abstract: Alternative milk products such as A2 milk are gaining popular stand within consumer market, for their healthy profile and expected greater digestibility characteristics. However, total mineral content and its bioaccessible profile have lacked in studies through the years, even more because of their relevance in public health. The present study aimed to evaluate the mineral profile of commercial A2 bovine milk (AT) and estimate the bio
accessibility of calcium, phosphorus and magnesium using the INFOGEST protocol. Non-A2 samples (NAT) were evaluated for comparison purpose. The determination of Ca, Mg, Na and K was performed by FAAS and total P was quantified by colorimetric method. Total protein content was determined by Kjeldahl method. Free amino acids were quantified by OPA method along the in vitro digestion stages. Total content of Ca, Na and P exhibited
equivalent results between samples, although A2 milk showed elevated levels of total Mg and K in the analyzed batches. AT showed protein content equivalent to NAT. In addition, levels of free NH2 were observed 2 times higher in AT, during the first hour of pancreatic phase in the intestinal digestion. Bioaccessibility of Ca showed equivalent percentages for AT (12–42 %) and NAT (10–39 %). The observed low values were possibly derived from interferences with saturated fatty acids and standardized electrolytes during digestion. Similar amounts of
bioaccessible Mg were found for all milk samples (35–97 %), while A2 samples evidenced percentages of bio accessible P exceeding 60 % across the three batches. Despite the health benefits associated to A2 milk, the study did not evidence clear distinction from non-A2 milk in terms of enhanced essential mineral solubility in digestive tract simulation, considering the association of greater digestibility expected for A2 milk

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