Agradecimentos: S.A.F. was the recipient of a post-graduation (Master degree) scholarship from CAPES/Brazil. This work was supported by FAPESP/Brazil (Proc. N. 95/0355-3). We are indebted to Dr Donald M. Bers for his valuable discussion and suggestions. We are also grateful to Mr Gilson B. Maia Jr, Ms Karen Silva, Ms Fabiana Kuhne, Ms Rubia Francchi and Ms Elisangela Oliveira for technical assistance Ver menos

Abstract: Post-rest contractile response was studied in isolated ventricular muscle from rats aged 1 to 90 days. Amplitude of rapid cooling contractures (RCC) was taken as an index of the sarcoplasmic reticulum (SR) Ca2+content. We observed that: (a) developed tension (per cross-section area) increased with age; (b) time to peak twitch force and relaxation half-time decreased from 87±6 to 56±2 ms and from 68±6 to 36±1 ms, respectively, from the neonatal period to adulthood; (c) post-rest twitch potentiation was observed at all ages, with greater relative potentiation in younger preparations, although relative potentiation of [Ca2+]itransient amplitude was similar in young and adult isolated ventricular myocytes; (d) rest did not significantly affect the amplitude of RCC in muscle or caffeine-evoked [Ca2+]itransients in myocytes at any studied age; (e) favoring Ca2+efflux via Na+–Ca2+exchange (NCX) during rest reversed twitch potentiation and caused a similar decrease in RCC amplitude (~40%) at all ages; (f) stimulation of Ca2+influx via NCX during rest increased RCC amplitude (~40%) only in immature preparations. However, when this procedure was repeated after partial SR Ca2+depletion, increase in RCC amplitude was not significantly age-dependent. We conclude that post-rest twitch potentiation is already present early after birth and does not require rest-dependent changes in SR Ca2+content at any studied age. Our results suggest that NCX is close to equilibrium during rest in both adult and developing rat myocardium, and does not seem to mediate diastolic net Ca2+fluxes which may affect the SR Ca2+content Ver menos