High concentrations of cannabidiol induce neurotoxicity in neurosphere culture system
Simone A. A. Romariz, Viviam Sanabria, Karina Ribeiro da Silva, Miguel L. Quintella, Bruna A. G. de Melo, Marimélia Porcionatto, Danilo Candido de Almeida, Beatriz M. Longo
ARTIGO
Inglês
Agradecimentos: This work was supported by FAPESP (#2022/00249-8), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (CAPES; Finance Code 001), and Conselho Nacional de Desenvolvimento Científico e Tecnológico. SR (2017/05242-3) and VS (2022/10520-0) receive post-doctoral...
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Agradecimentos: This work was supported by FAPESP (#2022/00249-8), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (CAPES; Finance Code 001), and Conselho Nacional de Desenvolvimento Científico e Tecnológico. SR (2017/05242-3) and VS (2022/10520-0) receive post-doctoral fellowships from the São Paulo Research Foundation (FAPESP)
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Abstract: Recent studies have demonstrated that cannabinoids are potentially effective in the treatment of various neurological conditions, and cannabidiol (CBD), one of the most studied compounds, has been proposed as a non-toxic option. However, the adverse effects of CBD on neurodevelopmental...
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Abstract: Recent studies have demonstrated that cannabinoids are potentially effective in the treatment of various neurological conditions, and cannabidiol (CBD), one of the most studied compounds, has been proposed as a non-toxic option. However, the adverse effects of CBD on neurodevelopmental processes have rarely been studied in cell culture systems. To better understand CBD’s influence on neurodevelopment, we exposed neural progenitor cells (NPCs) to different concentrations of CBD (1 µM, 5 µM, and 10 µM). We assessed the morphology, migration, differentiation, cell death, and gene expression in 2D and 3D bioprinted models to stimulate physiological conditions more effectively. Our results showed that CBD was more toxic at higher concentrations (5 µM and 10 µM) and affected the viability of NPCs than at lower concentrations (1 µM), in both 2D and 3D models. Moreover, our study revealed that higher concentrations of CBD drastically reduced the size of neurospheres and the number of NPCs within neurospheres, impaired the morphology and mobility of neurons and astrocytes after differentiation, and reduced neurite sprouting. Interestingly, we also found that CBD alters cellular metabolism by influencing the expression of glycolytic and ß-oxidative enzymes in the early and late stages of metabolic pathways. Therefore, our study demonstrated that higher concentrations of CBD promote important changes in cellular functions that are crucial during CNS development
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2022/00249-8
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
001
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
2017/05242-3; 2022/10520-0
Fechado
High concentrations of cannabidiol induce neurotoxicity in neurosphere culture system
Simone A. A. Romariz, Viviam Sanabria, Karina Ribeiro da Silva, Miguel L. Quintella, Bruna A. G. de Melo, Marimélia Porcionatto, Danilo Candido de Almeida, Beatriz M. Longo
High concentrations of cannabidiol induce neurotoxicity in neurosphere culture system
Simone A. A. Romariz, Viviam Sanabria, Karina Ribeiro da Silva, Miguel L. Quintella, Bruna A. G. de Melo, Marimélia Porcionatto, Danilo Candido de Almeida, Beatriz M. Longo
Fontes
Neurotoxicity research (Fonte avulsa) |