Agradecimentos: This research was funded by the Brazilian Government’s Ministry of Health PRONON initiative (NUP: 25000.192875/2019-78), Brazilian research financial institution, the National Council for Scientific and Technological Development (CNPq) (308276/2019-1), E.F.d.M. is a research fellow supported by Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP (2022/00994-5) Ver menos

Abstract: Advances in the development of pharmacological treatment in oral cancer require tumor models capable of simulating the complex biology of the tumor microenvironment. The spread of three-dimensional models has changed the scenery of in vitro cell culture techniques, contributing to translational oncology. Still, the full extent of their application in preclinical drug trials is yet to be understood. Therefore, the present scoping review protocol was established to screen the literature on using three-dimensional cell culture models in drug-testing assays in the context of oral cancer. This scoping review will be conducted based on the guidelines established by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Review guidelines (PRISMA-ScR). We will search the PubMed/Medline, Web of Science, Scopus, and Embase databases, as well as the gray literature, including peer-reviewed research articles involving 3D models applied to drug-assessment assays in oral cancer published from 1 March 2013 until 1 March 2023. Data will be charted, and findings will be described according to the predetermined questions of interest. We will present these findings in a narrative manner Ver menos