Abstract: It was prepared the graphene oxide (GO) sheets by suspension of GO in ultrapure deionized water or in Pluronic F-68 using a ultrasonicator bath. Total characterization of GO sheets was carried out. The results on suspension of GO in water showed excellent growth and cell adhesion. GO/Pluronic F-68 platform for the growth and adhesion of adipose-derived stem cells (ASCs) exhibited excellent properties also for these processes. In vivo study with GO suspended in water (100 µg mL-1) on Fisher 344 rats via i.p. administration showed low toxicity. In was also prepared three different scaffolds, such as, graphene oxide/poly(e-caprolactone) (GO/PCL), graphene oxide/Pegylated amino derivative (GO/PEG-NH2) and graphene oxide/Pegylated amino derivative/Poly(e-caprolactone)(GO/PEG-NH2/PCL) for the growth and adhesion of MSCs and they exhibited excellent MSCs adhesion properties. In vivo study with GO suspended in water on Fisher 344 rats via i.p. administration showed no toxicity up to 100 µg µL-1, in spite of part of these GOs accumulated in the intraperitoneal cavity at the first hours. However, with these scaffolds no deposition occurred. Then, taking together, the data suggest the great potential of GO scaffolds to MSCs, as well as, new material for treatment several urological diseases. The application of theses GO scaffold is actually in progress since urethral stricture and hypospadias are treatment restricted, since, substitution of the unhealthy urethra with tissue from other origins (bladder, skin or buccal mucosa) are limited Ver menos