Agradecimentos: The authors are thanking to Brejeiro food products (Orlândia, Brazil), for the donation of the soybean seeds (variety BRS232) employed in our study. This work was financial supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grant numbers 2014/50867–3, 2017/50085–3, 2018/25207–0, 2019/00063–9, 2019/24445–8, and 2020/08543–7), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Financial code 001, and also the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasília, Brazil) [grant number 303231/2020–3] Ver menos

Abstract: Nanoparticles (NPs) are currently found in the world in the form of natural colloids and volcanic ash, as well as in anthropogenic sources, such as nanofertilizers; however, in the literature, there is still a lack of toxicological evidence, risk assessment, and regulations about the use and environmental impact of NPs in the agroindustrial system. Therefore, the aim of this work was to evaluate alterations caused by the presence of AgNPs during the development of soybean plants. The BRS232 non-transgenic (NT) soybean plant and 8473RR (TRR) and INTACTA RR2 PRO (TIntacta) transgenic soybean plants were irrigated for 18 days under controlled conditions with deionized water (control), AgNPs, and AgNO3. The isotopes 107Ag+, 55Mn+, 57Fe+, 63Cu+, and 64Zn+ were mapped in leaves, using 13C+ as an internal standard (IS), and carried out using a laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) technique with a Nd:YAG (213 nm) laser source in the imagagin mode using the LA-iMageS software and also Mathlab. Leaf images showed a low Ag translocation, indicated by the basal signal of this ion. Additionally, the presence of Ag in the ionic form and as NPs altered the homeostasis of 112Cd+, 64Zn+, 55Mn+, 63Cu+, and 57Fe+ in different ways. Quantitative image analysis was performed for Cu. The behavior of TRR and TIntacta plants was different in the presence of ionic silver or AgNPs, confirming that the metabolism of these two plants, despite both being transgenic, are different. Through the images, it was observed that the response of plants was different in the face of the same stress conditions during their development Ver menos