Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding
Geovani Luciano de Oliveira, Guilherme Francio Niederauer, Fernanda Ancelmo de Oliveira, Cinthia Souza Rodrigues, José Luiz Hernandes, Anete Pereira de Souza, Mara Fernandes Moura
ARTIGO
Inglês
Agradecimentos: This study was funded by the São Paulo Research Foundation (FAPESP) grants 2018/ 13539 -9 and 2020/12938-7 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant 404041/2021-3. Coordination for the Improvement of Higher Education Personnel (CAPES) provided...
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Agradecimentos: This study was funded by the São Paulo Research Foundation (FAPESP) grants 2018/ 13539 -9 and 2020/12938-7 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant 404041/2021-3. Coordination for the Improvement of Higher Education Personnel (CAPES) provided fellowships to GLO (grant 88887.479802/2020-00), GFN (grant 88887.513247/2020-00), and CSR (grant 88882.317454/2019-01). FAO received a PostDoctoral fellowship from the São Paulo Research Foundation (FAPESP) (grant 2018/18527–9). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript
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Abstract: In the 1940s, the Agronomic Institute of Campinas (IAC) started a grapevine breeding program to develop new cultivars adapted to the tropical and subtropical regions of Brazil. More than 2,000 crosses were carried out over 50 years, using 850 varieties as parents. However, among the...
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Abstract: In the 1940s, the Agronomic Institute of Campinas (IAC) started a grapevine breeding program to develop new cultivars adapted to the tropical and subtropical regions of Brazil. More than 2,000 crosses were carried out over 50 years, using 850 varieties as parents. However, among the thousands of hybrids developed by the program, only 130 are still maintained in the IAC grapevine germplasm collection. Little is known about their genetic makeup and usefulness for current breeding programs. In this study, we genotyped 130 Brazilian grape hybrids at 21 polymorphic microsatellite markers to evaluate the genetic diversity and population structure of the hybrids and verified their disclosed pedigrees. The results showed that the hybrid collection is highly diverse, with an expected heterozygosity (HE) of 0.80 and an observed heterozygosity (Ho) of 0.78. Strong structure in three subgroups based mainly on the usage and combination of parental groups was revealed by STRUCTURE software and confirmed by discriminant analysis of principal components (DAPC). Through molecular profiling analysis, fourteen synonyms, one homonym and one duplicate were identified. Parentage analysis confirmed 24 full parentages, as well as 33 half-kinships. In addition, 18 pedigrees were invalidated, and seven mislabeling events were identified. No compatible parent was identified for 33% of the IAC hybrids, highlighting severe genetic erosion in the IAC germplasm. The molecular characterization of the breeding hybrid bank collection contributes to our understanding of the genetic basis of the varieties, guiding the efficient utilization of available genetic diversity. Together, our results could be applied to other breeding programs and assist in the selection of parents, management of the breeding collection, and conservation of grapevine genetic resources
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2018/13539-9; 2018/18527–9; 2020/12938-7
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
404041/2021-3
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
88882.317454/2019-01; 88887.513247/2020-00; 88887.479802/2020-00
Fechado
Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding
Geovani Luciano de Oliveira, Guilherme Francio Niederauer, Fernanda Ancelmo de Oliveira, Cinthia Souza Rodrigues, José Luiz Hernandes, Anete Pereira de Souza, Mara Fernandes Moura
Genetic diversity, population structure and parentage analysis of Brazilian grapevine hybrids after half a century of genetic breeding
Geovani Luciano de Oliveira, Guilherme Francio Niederauer, Fernanda Ancelmo de Oliveira, Cinthia Souza Rodrigues, José Luiz Hernandes, Anete Pereira de Souza, Mara Fernandes Moura
Fontes
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Scientia horticulturae v. 311, n. art. 111825, Mar. 2023 |