Phenotypic changes in low-density lipoprotein particles as markers of adverse clinical outcomes in Covid-19
Helison Rafael P. Carmo, Marcos Y. Yoshinaga, Alejandro Rosell Castillo, Adriano Britto Chaves-Filho, Isabella Bonilha, Joaquim Barreto, Stéfanie Primon Muraro, Gabriela Fabiano de Souza, Gustavo Gastão Davanzo, Maurício W. Perroud, Kishal Lukhna, Mpiko Ntsekhe, Sean Davidson, Licio A. Velloso,...
Helison Rafael P. Carmo, Marcos Y. Yoshinaga, Alejandro Rosell Castillo, Adriano Britto Chaves-Filho, Isabella Bonilha, Joaquim Barreto, Stéfanie Primon Muraro, Gabriela Fabiano de Souza, Gustavo Gastão Davanzo, Maurício W. Perroud, Kishal Lukhna, Mpiko Ntsekhe, Sean Davidson, Licio A. Velloso, Wilson Nadruz, Luiz Sérgio F. Carvalho, Miguel Sáinz-Jaspeado, Alessandro S. Farias, José Luiz Proença-Módena, Pedro M. Moraes-Vieira, Sotirios K. Karathanasis, Derek Yellon, Sayuri Miyamoto, Alan T. Remaley, Andrei C. Sposito
ARTIGO
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Agradecimentos: We thank Clarissa Jones for assisting with oxidized LDL kits from company Mercodia (Uppsala, Sweden). H.R.P.C. and A.R.C. were awarded a scholarship from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grants: 2018/17792–0 and 2020/12649–5, respectively. A.C.S. was...
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Agradecimentos: We thank Clarissa Jones for assisting with oxidized LDL kits from company Mercodia (Uppsala, Sweden). H.R.P.C. and A.R.C. were awarded a scholarship from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grants: 2018/17792–0 and 2020/12649–5, respectively. A.C.S. was supported by a Research Career Awards grant (304,257/2021–4) from the Brazilian National Research Council (CNPq). S.M. was supported by FAPESP grant (13/07937–8). J.L.P.-M. was supported by FAPESP (2016/00194–8, 2020/04558–0), CNPq (305,628/2020–8) and "Fundo de Apoio ao Ensino, Pesquisa e Extensão" of UNICAMP (FAEPEX-UNICAMP 2266/20)
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Abstract: Low-density lipoprotein (LDL) plasma concentration decline is a biomarker for acute inflammatory diseases, including coronavirus disease-2019 (COVID-19). Phenotypic changes in LDL during COVID-19 may be equally related to adverse clinical outcomes. METHODS: Individuals hospitalized due to...
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Abstract: Low-density lipoprotein (LDL) plasma concentration decline is a biomarker for acute inflammatory diseases, including coronavirus disease-2019 (COVID-19). Phenotypic changes in LDL during COVID-19 may be equally related to adverse clinical outcomes. METHODS: Individuals hospitalized due to COVID-19 (n = 40) were enrolled. Blood samples were collected on days 0, 2, 4, 6, and 30 (D0, D2, D4, D6, and D30). Oxidized LDL (ox-LDL), and lipoprotein-associated phospholipase A2 (Lp-PLA2) activity were measured. In a consecutive series of cases (n = 13), LDL was isolated by gradient ultracentrifugation from D0 and D6 and was quantified by lipidomic analysis. Association between clinical outcomes and LDL phenotypic changes was investigated. RESULTS: In the first 30 days, 42.5% of participants died due to Covid-19. The serum ox-LDL increased from D0 to D6 (p < 0.005) and decreased at D30. Moreover, individuals who had an ox-LDL increase from D0 to D6 to over the 90th percentile died. The plasma Lp-PLA2 activity also increased progressively from D0 to D30 (p < 0.005), and the change from D0 to D6 in Lp-PLA2 and ox-LDL were positively correlated (r = 0.65, p < 0.0001). An exploratory untargeted lipidomic analysis uncovered 308 individual lipids in isolated LDL particles. Paired-test analysis from D0 and D6 revealed higher concentrations of 32 lipid species during disease progression, mainly represented by lysophosphatidyl choline and phosphatidylinositol. In addition, 69 lipid species were exclusively modulated in the LDL particles from non-survivors as compared to survivors. CONCLUSIONS: Phenotypic changes in LDL particles are associated with disease progression and adverse clinical outcomes in COVID-19 patients and could serve as a potential prognostic biomarker
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2013/07937–8; 2016/00194–8; 2018/17792–0; 2020/12649–5; 2020/04558–0
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
305628/2020–8; 304257/2021–4
Fechado
Phenotypic changes in low-density lipoprotein particles as markers of adverse clinical outcomes in Covid-19
Helison Rafael P. Carmo, Marcos Y. Yoshinaga, Alejandro Rosell Castillo, Adriano Britto Chaves-Filho, Isabella Bonilha, Joaquim Barreto, Stéfanie Primon Muraro, Gabriela Fabiano de Souza, Gustavo Gastão Davanzo, Maurício W. Perroud, Kishal Lukhna, Mpiko Ntsekhe, Sean Davidson, Licio A. Velloso,...
Helison Rafael P. Carmo, Marcos Y. Yoshinaga, Alejandro Rosell Castillo, Adriano Britto Chaves-Filho, Isabella Bonilha, Joaquim Barreto, Stéfanie Primon Muraro, Gabriela Fabiano de Souza, Gustavo Gastão Davanzo, Maurício W. Perroud, Kishal Lukhna, Mpiko Ntsekhe, Sean Davidson, Licio A. Velloso, Wilson Nadruz, Luiz Sérgio F. Carvalho, Miguel Sáinz-Jaspeado, Alessandro S. Farias, José Luiz Proença-Módena, Pedro M. Moraes-Vieira, Sotirios K. Karathanasis, Derek Yellon, Sayuri Miyamoto, Alan T. Remaley, Andrei C. Sposito
Phenotypic changes in low-density lipoprotein particles as markers of adverse clinical outcomes in Covid-19
Helison Rafael P. Carmo, Marcos Y. Yoshinaga, Alejandro Rosell Castillo, Adriano Britto Chaves-Filho, Isabella Bonilha, Joaquim Barreto, Stéfanie Primon Muraro, Gabriela Fabiano de Souza, Gustavo Gastão Davanzo, Maurício W. Perroud, Kishal Lukhna, Mpiko Ntsekhe, Sean Davidson, Licio A. Velloso,...
Helison Rafael P. Carmo, Marcos Y. Yoshinaga, Alejandro Rosell Castillo, Adriano Britto Chaves-Filho, Isabella Bonilha, Joaquim Barreto, Stéfanie Primon Muraro, Gabriela Fabiano de Souza, Gustavo Gastão Davanzo, Maurício W. Perroud, Kishal Lukhna, Mpiko Ntsekhe, Sean Davidson, Licio A. Velloso, Wilson Nadruz, Luiz Sérgio F. Carvalho, Miguel Sáinz-Jaspeado, Alessandro S. Farias, José Luiz Proença-Módena, Pedro M. Moraes-Vieira, Sotirios K. Karathanasis, Derek Yellon, Sayuri Miyamoto, Alan T. Remaley, Andrei C. Sposito
Fontes
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Molecular genetics and metabolism v. 138, n. 4, n. art. 107552, Apr. 2023 |