Agradecimentos: This study is partially supported by the São Paulo Research Foundation (2020/05231-4 to V.A.R.B., 2020/07087-8 and 2022/07353-5 to M.H.R.B; 2019/17238-6 to B.E.N., 2020/10436-4 to R.C.C.), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (307471/2021-7 to V.A.R.B.), and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance code 001). Graphical abstract and figures were designed using the web interface BioRender® Ver menos

Abstract: Polypyrrole (PPy) is one of the most studied conductive polymers due to its electrical conductivity and biological properties, which drive the possibility of numerous applications in the biomedical area. The physical-chemical features of PPy allow the manufacture of biocompatible devices, enhancing cell adhesion and proliferation. Furthermore, owing to the electrostatic interactions between the negatively charged bacterial cell wall and the positive charges in the polymer structure, PPy films can perform an effective antimicrobial activity. PPy is also frequently associated with biocompatible agents and antimicrobial compounds to improve the biological response. Thus, this comprehensive review appraised the available evidence regarding the PPy-based films deposited on metallic implanted devices for biomedical applications. We focus on understanding key concepts that could influence PPy attributes regarding antimicrobial effect and cell behavior under in vitro and in vivo settings. Furthermore, we unravel the several agents incorporated into the PPy film and strategies to improve its functionality. Our findings suggest that incorporating other elements into the PPy films, such as antimicrobial agents, biomolecules, and other biocompatible polymers, may improve the biological responses. Overall, the basic properties of PPy, when combined with other composites, electrostimulation techniques, or surface treatment methods, offer great potential in biocompatibility and/or antimicrobial activities. However, challenges in synthesis standardization and potential limitations such as low adhesion and mechanical strength of the film must be overcome to improve and broaden the application of PPy film in biomedical devices Ver menos