Enzymatic hydrolysis of rutin : evaluation of kinetic parameters and anti-proliferative, mutagenic and anti-mutagenic effects
Mariana Alves Sobreiro, Adriana Della Torre, Maria Elisa Melo Branco de Araújo, Paula Renata Bueno Campos Canella, João Ernesto de Carvalho, Patrícia de Oliveira Carvalho, Ana Lucia Tasca Gois Ruiz
ARTIGO
Inglês
Agradecimentos: The authors thank the Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA/Unicamp) for the infrastructure. This research was funded by the São Paulo Research Foundation (FAPESP, grant numbers #2013/13196-0, #2011/12394-8, #2011/03540-0, #2011/01114-4,...
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Agradecimentos: The authors thank the Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA/Unicamp) for the infrastructure. This research was funded by the São Paulo Research Foundation (FAPESP, grant numbers #2013/13196-0, #2011/12394-8, #2011/03540-0, #2011/01114-4, #2009/09224-3 and #2009/08689-2), and the Coordination for the Improvement of Higher Education Personnel (CAPES, Financing Code 001). The authors J.E.d.C., P.d.O.C. and A.L.T.G.R. (#313440/2019-0) also thank the National Council for Scientific and Technological Development CNPq for their research productivity fellowship
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Abstract: The bioavailability of glucoside flavonoids is influenced by the nature of the sugar, glucosides being absorbed faster than rhamnoglucosides, for example. One strategy to enhance the bioavailability is enzymatic hydrolysis. In this study, some kinetic parameters of hesperidinase-mediated...
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Abstract: The bioavailability of glucoside flavonoids is influenced by the nature of the sugar, glucosides being absorbed faster than rhamnoglucosides, for example. One strategy to enhance the bioavailability is enzymatic hydrolysis. In this study, some kinetic parameters of hesperidinase-mediated hydrolysis of rutin were evaluated using an UHPLC/QTOF-MSE analysis of the products of a bioconversion reaction. The resulting hydrolyzed rutins (after 4, 8 and 12 h of reaction) were submitted to anti-proliferative and Cytokinesis-Block Micronucleus (CBMN) assays in CHO-K1 cells. In the hesperidinase-mediated hydrolysis, the final concentration of quercetin-3-O-glucoside (Q3G) was directly proportional to the rutin concentration and inversely proportional to the reaction time. At an anti-proliferative concentration (2.5 µg/mL), hydrolyzed rutin derivatives did not show a mutagenic effect, except for the sample with a higher content of Q3G (after 4 h of the enzymatic hydrolysis of rutin). Moreover, the higher Q3G content in hydrolyzed rutin protected the CHO-K1 cells 92% of the time against methyl methanesulfonate-induced mutagenic damage. These results suggested that the anti-mutagenic effect of hydrolyzed rutin might be related to antioxidant and cell death induction. Presenting a good lipophilicity/hydrophilicity ratio, together with antioxidant and anti-mutagenic activities, the hesperidinase-mediated hydrolyzed rutin seemed to be a promisor raw material for the development of food supplements
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2009/09224-3; 2009/08689-2; 2011/12394-8; 2011/03540-0; 2011/01114-4; 2013/13196-0
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
001
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
Aberto
DOI: https://doi.org/10.3390/life13020549
Texto completo: https://www.mdpi.com/2075-1729/13/2/549
Enzymatic hydrolysis of rutin : evaluation of kinetic parameters and anti-proliferative, mutagenic and anti-mutagenic effects
Mariana Alves Sobreiro, Adriana Della Torre, Maria Elisa Melo Branco de Araújo, Paula Renata Bueno Campos Canella, João Ernesto de Carvalho, Patrícia de Oliveira Carvalho, Ana Lucia Tasca Gois Ruiz
Enzymatic hydrolysis of rutin : evaluation of kinetic parameters and anti-proliferative, mutagenic and anti-mutagenic effects
Mariana Alves Sobreiro, Adriana Della Torre, Maria Elisa Melo Branco de Araújo, Paula Renata Bueno Campos Canella, João Ernesto de Carvalho, Patrícia de Oliveira Carvalho, Ana Lucia Tasca Gois Ruiz
Fontes
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Life v. 13, n. 2, n. art. 549, 2023 |