Metabolomics approach reveals important glioblastoma plasma biomarkers for tumor biology
Adriana C. Ferrasi, Ricardo Puttini, Aline F. Galvani, Pedro T. Hamamoto Filho, Jeany Delafiori, Victoria D. Argente, Arthur N. de Oliveira, Flávia L. Dias-Audibert, Rodrigo R. Catharino, Octavio C. Silva, Marco A. Zanini, Gabriel A. Kurokawa, Estela O. Lima
ARTIGO
Inglês
Agradecimentos: The Laboratory of Molecular Analysis and Neuro-oncology and Innovare Laboratory thank the Institutional Scientific Initiation Scholarship Program/National Council for Scientific and Technological Development (PIBIC-CNPq) for the fellowship provided to O.C.S. (process no....
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Agradecimentos: The Laboratory of Molecular Analysis and Neuro-oncology and Innovare Laboratory thank the Institutional Scientific Initiation Scholarship Program/National Council for Scientific and Technological Development (PIBIC-CNPq) for the fellowship provided to O.C.S. (process no. 2862/4-2021), to G.A.K. (process no. 534/1-2020), and to V.D.A (process no. 2870/4-2021). We also thank the Coordination of Superior Level Staff Improvement- Brazil (CAPES) for the fellowships to A.N.O. (Code 88887.513974/2020-00) and to A.F.G. (Code 88887.670079/2022-00) and the São Paulo Research Foundation (FAPESP) for the fellowship to R. R. C. (process no. 2018/14657-5) and J. D. (process no. 2019/05718-3). The funders had no role in the study design, data collection and interpretation, or decision to submit the work for publication
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Abstract: Glioblastoma (GB) is the most aggressive and frequent primary malignant tumor of the central nervous system and is associated with poor overall survival even after treatment. To better understand tumor biochemical alterations and broaden the potential targets of GB, this study aimed to...
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Abstract: Glioblastoma (GB) is the most aggressive and frequent primary malignant tumor of the central nervous system and is associated with poor overall survival even after treatment. To better understand tumor biochemical alterations and broaden the potential targets of GB, this study aimed to evaluate differential plasma biomarkers between GB patients and healthy individuals using metabolomics analysis. Plasma samples from both groups were analyzed via untargeted metabolomics using direct injection with an electrospray ionization source and an LTQ mass spectrometer. GB biomarkers were selected via Partial Least Squares Discriminant and Fold-Change analyses and were identified using tandem mass spectrometry with in silico fragmentation, consultation of metabolomics databases, and a literature search. Seven GB biomarkers were identified, some of which were unprecedented biomarkers for GB, including arginylproline (m/z 294), 5-hydroxymethyluracil (m/z 143), and N-acylphosphatidylethanolamine (m/z 982). Notably, four other metabolites were identified. The roles of all seven metabolites in epigenetic modulation, energy metabolism, protein catabolism or folding processes, and signaling pathways that activate cell proliferation and invasion were elucidated. Overall, the findings of this study highlight new molecular targets to guide future investigations on GB. These molecular targets can also be further evaluated to derive their potential as biomedical analytical tools for peripheral blood samples
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
534/1-2020; 2862/4-2021; 2870/4-2021
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
88887.513974/2020-00; 88887.670079/2022-00
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2018/14657-5; 2019/05718-3
Aberto
DOI: https://doi.org/10.3390/ijms24108813
Texto completo: https://www.mdpi.com/1422-0067/24/10/8813
Metabolomics approach reveals important glioblastoma plasma biomarkers for tumor biology
Adriana C. Ferrasi, Ricardo Puttini, Aline F. Galvani, Pedro T. Hamamoto Filho, Jeany Delafiori, Victoria D. Argente, Arthur N. de Oliveira, Flávia L. Dias-Audibert, Rodrigo R. Catharino, Octavio C. Silva, Marco A. Zanini, Gabriel A. Kurokawa, Estela O. Lima
Metabolomics approach reveals important glioblastoma plasma biomarkers for tumor biology
Adriana C. Ferrasi, Ricardo Puttini, Aline F. Galvani, Pedro T. Hamamoto Filho, Jeany Delafiori, Victoria D. Argente, Arthur N. de Oliveira, Flávia L. Dias-Audibert, Rodrigo R. Catharino, Octavio C. Silva, Marco A. Zanini, Gabriel A. Kurokawa, Estela O. Lima
Fontes
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International journal of molecular sciences v. 24, n. 10, n. art. 8813, 2023 |