Influence of the degree of hydrolysis and type of enzyme on antioxidant activity of okara protein hydrolysates
Melissa Ferreira Sbroggio, Marina Silveira Montilha, Vitória Ribeiro Garcia de Figueiredo, Sandra Regina Georgetti, Louise Emy Kurozawa
ARTIGO
Português
Agradecimentos: The authors acknowledge the financial support of the the Araucaria Foundation for Supporting Scientific and Technological Development of Paraná (160/2014) and the Brazilian National Council for Scientific and Technological Development CNPq (473117/2013-4). LEK is an Araucaria...
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Agradecimentos: The authors acknowledge the financial support of the the Araucaria Foundation for Supporting Scientific and Technological Development of Paraná (160/2014) and the Brazilian National Council for Scientific and Technological Development CNPq (473117/2013-4). LEK is an Araucaria Foundation research fellow
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Abstract: The objective of this work was to evaluate the antioxidant activity of protein hydrolysates obtained by the enzymatic hydrolysis of okara using an endopeptidase (Alcalase) and exopeptidase (Flavourzyme). The reaction was monitored by the pH-stat procedure in which five aliquots were...
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Abstract: The objective of this work was to evaluate the antioxidant activity of protein hydrolysates obtained by the enzymatic hydrolysis of okara using an endopeptidase (Alcalase) and exopeptidase (Flavourzyme). The reaction was monitored by the pH-stat procedure in which five aliquots were collected during the hydrolysis by each enzyme, corresponding to different degrees of hydrolysis (DH). The antioxidant activities of the aliquots were evaluated by the ABTS, DPPH and FRAP methods. For the hydrolysates obtained using Alcalase, the antioxidant activities increased from: 68.6 to 99.5% (ABTS), 14.5 to 17.7% (DPPH) and 222.6 to 684.9 µM Trolox (FRAP), when the DH varied from 0 to 33.6%. With respect to Flavourzyme, the results were: 67.2 to 88.2% (ABTS), 9.5 to 18.5% (DPPH) and 168.0 to 360.3 ?M Trolox (FRAP), when the DH increased up to 5.8%. The results showed that the protein hydrolysates had antioxidant capacities, which were influenced by the degree of hydrolysis and the type of enzyme
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FUNDAÇÃO ARAUCÁRIA DE APOIO AO DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO DO ESTADO DO PARANÁ - FA
160/2014
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
473117/2013-4
Aberto
Influence of the degree of hydrolysis and type of enzyme on antioxidant activity of okara protein hydrolysates
Melissa Ferreira Sbroggio, Marina Silveira Montilha, Vitória Ribeiro Garcia de Figueiredo, Sandra Regina Georgetti, Louise Emy Kurozawa
Influence of the degree of hydrolysis and type of enzyme on antioxidant activity of okara protein hydrolysates
Melissa Ferreira Sbroggio, Marina Silveira Montilha, Vitória Ribeiro Garcia de Figueiredo, Sandra Regina Georgetti, Louise Emy Kurozawa
Fontes
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Food science and technology = Ciência e tecnologia de alimentos (Fonte avulsa) |