Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome
Gabriela Vaz Meirelles, Daniel Carlos Ferreira Lanza, Júlio César da Silva, Jéssica Santana Bernachi, Adriana Franco Paes Leme, Jörg Kobarg
ARTIGO
Inglês
Agradecimentos: We thank Maria Eugênia Camargo, Thaís C. D. Dombroski, Bianca A. Pauletti, Carla C. Judice, Jackeline de Lima, and Janine S. Sabino for technical assistance. We are also grateful to Dr. Kleber G. Franchini for help in providing the THP1 cells, and to Dr. Sara T. O. Saad for the use...
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Agradecimentos: We thank Maria Eugênia Camargo, Thaís C. D. Dombroski, Bianca A. Pauletti, Carla C. Judice, Jackeline de Lima, and Janine S. Sabino for technical assistance. We are also grateful to Dr. Kleber G. Franchini for help in providing the THP1 cells, and to Dr. Sara T. O. Saad for the use of the confocal microscope. This work was supported by Fundação de Amparo à Pesquisa do Estado São Paulo (FAPESP), Conselho Nacional de Pesquisa e Desenvolvimento (CNPq), and the Associação Brasileira de Tecnologia de Luz Síncrotron (ABTLuS)
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Abstract: Physical protein protein interactions are fundamental to all biological processes and are organized in complex networks One branch of the kinome network is the evolutionarily conserved NIMA-related serine/threonine kinases (Neks) Most of the 11 mammalian Neks studied so far are related to...
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Abstract: Physical protein protein interactions are fundamental to all biological processes and are organized in complex networks One branch of the kinome network is the evolutionarily conserved NIMA-related serine/threonine kinases (Neks) Most of the 11 mammalian Neks studied so far are related to cell cycle regulation and due to association with diverse human pathologies, Neks are promising chemotherapeutic targets Human Nek6 was associated to carcinogenesis but its interacting partners and signaling pathways remain elusive Here we introduce hNek6 as a highly connected member in the human kinase interactome In a more global context, we performed a broad data bank comparison based on degree distribution analysis and found that the human kinome is enriched in hubs Our networks include a broad set of novel hNek6 interactors as identified by our yeast two hybrid screens classified into 18 functional categories All of the tested interactions were confirmed and the majority of tested substrates were phosphorylated in vitro by hNek6 Notably, we found that hNek6 N-terminal is important to mediate the interactions with its partners Some novel interactors also colocalized with hNek6 and gamma-tubulin in human cells, pointing to a possible centrosomal interaction The interacting proteins link hNek6 to novel pathways, for example, Notch signaling and actin cytoskeleton regulation, or give new insights on how hNek6 may regulate previously proposed pathways such as cell cycle regulation, DNA repair response, and NF-kappa B signaling Our findings open new perspectives in the study of hNek6 role in cancer by analyzing its novel interactions in specific pathways in tumor cells which may provide important implications for drug design and cancer therapy
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
Fechado
DOI: https://doi.org/10.1021/pr100562w
Texto completo: https://pubs.acs.org/doi/10.1021/pr100562w
Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome
Gabriela Vaz Meirelles, Daniel Carlos Ferreira Lanza, Júlio César da Silva, Jéssica Santana Bernachi, Adriana Franco Paes Leme, Jörg Kobarg
Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome
Gabriela Vaz Meirelles, Daniel Carlos Ferreira Lanza, Júlio César da Silva, Jéssica Santana Bernachi, Adriana Franco Paes Leme, Jörg Kobarg
Fontes
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Journal of proteome research Vol. 9, n. 12 (Dec., 2010), p. 6298-6316 |