Merulinic acid C overcomes gentamicin resistance in Enterococcus faecium
Fernanda Rodrigues-Costa, Juliano Slivinski, Laura P. Ióca, Ariane F. Bertonha, Rafael de Felício, Marcos Guilherme da Cunha, Paulo Vinicius da Mata Madeira, Ana C.G. Cauz, Daniel Maragno Trindade, Vítor F. Freire, Cristina Dislich Ropke, Ana Gales, Marcelo Brocchi, Antônio G. Ferreira, Frederico...
Fernanda Rodrigues-Costa, Juliano Slivinski, Laura P. Ióca, Ariane F. Bertonha, Rafael de Felício, Marcos Guilherme da Cunha, Paulo Vinicius da Mata Madeira, Ana C.G. Cauz, Daniel Maragno Trindade, Vítor F. Freire, Cristina Dislich Ropke, Ana Gales, Marcelo Brocchi, Antônio G. Ferreira, Frederico Gueiros-Filho, Daniela Barretto Barbosa Trivella, Roberto G.S. Berlinck, Andréa Dessen
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Agradecimentos: The authors thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) for financial support to R.G.S.B. (BIOTA-BIOprospecTA grant 2013/50228-8) and a PhD scholarship to L.P.I (2016/05133-7), CAPES for a MSc scholarship for J.S, a PhD scholarship to A.F.B., and CNPq for a...
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Agradecimentos: The authors thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) for financial support to R.G.S.B. (BIOTA-BIOprospecTA grant 2013/50228-8) and a PhD scholarship to L.P.I (2016/05133-7), CAPES for a MSc scholarship for J.S, a PhD scholarship to A.F.B., and CNPq for a PhD scholarship to V.F.F. We also acknowledge support to A.D. from the Laboratoire International Associé BACWALL (CNRS, France), and FAPESP grants 2011/52067-6 (BACWALL) and 2017/12436-9 (ANTIBIO-BAC). F.R.C. and P.V.M.M. were supported by Ph.D. fellowships 2015/19906-5 and 2014/11980-9, respectively, from FAPESP. A.C.G.C. was supported by Ph.D. fellowship 1521952 from CAPES. We acknowledge the Chemistry and Natural Product (LQPN), the Nuclear Magnetic Resonance (NMR) and the Bioassay (LBE) laboratories at the Brazilian Biosciences National Laboratory (LNBio), CNPEM, Campinas, Brazil for their technical and infrastructure support. We also wish to thank Prof. Dr. Elisandro R. Drechsler-Santos (Universidade Federal de Santa Catarina, UFSC) for the identification of the basidiomycete and Dr. Ivanilza Moreira de Andrade for A. occidentale species identification (a voucher was deposited in the Parnaíba Delta herbarium under number 1830)
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Abstract: Enterococci are gram-positive, widespread nosocomial pathogens that in recent years have developed resistance to various commonly employed antibiotics. Since finding new infection-control agents based on secondary metabolites from organisms has proved successful for decades, natural...
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Abstract: Enterococci are gram-positive, widespread nosocomial pathogens that in recent years have developed resistance to various commonly employed antibiotics. Since finding new infection-control agents based on secondary metabolites from organisms has proved successful for decades, natural products are potentially useful sources of compounds with activity against enterococci. Herein are reported the results of a natural product library screening based on a whole-cell assay against a gram-positive model organism, which led to the isolation of a series of anacardic acids identified by analysis of their spectroscopic data and by chemical derivatizations. Merulinic acid C was identified as the most active anacardic acid derivative obtained against antibiotic-resistant enterococci. Fluorescence microscopy analyses showed that merulinic acid C targets the bacterial membrane without affecting the peptidoglycan and causes rapid cellular ATP leakage from cells. Merulinic acid C was shown to be synergistic with gentamicin against Enterococcus faecium, indicating that this compound could inspire the development of new antibiotic combinations effective against drug-resistant pathogens
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2011/52067-6; 2013/50228-8; 2014/11980-9; 2015/19906-5; 2016/05133-7; 2017/12436-9
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
1521952
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
Fechado
Merulinic acid C overcomes gentamicin resistance in Enterococcus faecium
Fernanda Rodrigues-Costa, Juliano Slivinski, Laura P. Ióca, Ariane F. Bertonha, Rafael de Felício, Marcos Guilherme da Cunha, Paulo Vinicius da Mata Madeira, Ana C.G. Cauz, Daniel Maragno Trindade, Vítor F. Freire, Cristina Dislich Ropke, Ana Gales, Marcelo Brocchi, Antônio G. Ferreira, Frederico...
Fernanda Rodrigues-Costa, Juliano Slivinski, Laura P. Ióca, Ariane F. Bertonha, Rafael de Felício, Marcos Guilherme da Cunha, Paulo Vinicius da Mata Madeira, Ana C.G. Cauz, Daniel Maragno Trindade, Vítor F. Freire, Cristina Dislich Ropke, Ana Gales, Marcelo Brocchi, Antônio G. Ferreira, Frederico Gueiros-Filho, Daniela Barretto Barbosa Trivella, Roberto G.S. Berlinck, Andréa Dessen
Merulinic acid C overcomes gentamicin resistance in Enterococcus faecium
Fernanda Rodrigues-Costa, Juliano Slivinski, Laura P. Ióca, Ariane F. Bertonha, Rafael de Felício, Marcos Guilherme da Cunha, Paulo Vinicius da Mata Madeira, Ana C.G. Cauz, Daniel Maragno Trindade, Vítor F. Freire, Cristina Dislich Ropke, Ana Gales, Marcelo Brocchi, Antônio G. Ferreira, Frederico...
Fernanda Rodrigues-Costa, Juliano Slivinski, Laura P. Ióca, Ariane F. Bertonha, Rafael de Felício, Marcos Guilherme da Cunha, Paulo Vinicius da Mata Madeira, Ana C.G. Cauz, Daniel Maragno Trindade, Vítor F. Freire, Cristina Dislich Ropke, Ana Gales, Marcelo Brocchi, Antônio G. Ferreira, Frederico Gueiros-Filho, Daniela Barretto Barbosa Trivella, Roberto G.S. Berlinck, Andréa Dessen
Fontes
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Bioorganic chemistry Vol. 100 (July, 2020), n. art. 103921 |