Agradecimentos: This study was supported by the São Paulo Research Foundation (FAPESP) under grants 2019/10732-5 (CBC), 2020/07634-9 (DSC), 2020/11596-5 (IMP), and 2012/50533-2, in part by the Coordination for the Improvement of Higher Education Personnel (CAPES) under Finance Code 001 (HDR, CBC, DSC), and the Fellowship of Research Productivity (PQ2-CNPq) granted by the National Council for Scientific and Technological Development, number 301912/2019-0 (MFGN) Ver menos

Abstract: The aim of this study was to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in co-culture with in vitro-produced bovine embryos (IVPEs). The stability of five concentrations of MLVs (1.0, 1.25, 1.5, 1.75, and 2.0mM) produced using ultrapure water or embryonic culture medium with 24 or 48h of incubation at 38.5°C with 5% CO2 was assessed. In addition, the toxicity of MLVs and their modulation of the lipid profile of the plasma membrane of IVPEs were evaluated after 48h of co-culture. Both media allowed the production of MLVs. Incubation (24 and 48h) did not impair the MLV structure but affected the average diameter. The rate of blastocyst production was not reduced, demonstrating the nontoxicity of the MLVs even at 2.0mmol/L. The lipid profile of the embryos was different depending on the MLV concentration. In comparison with control embryos, embryos cultured with MLVs at 2.0mmol/L had a higher relative abundance of six lipid ions (m/z 720.6, 754.9, 759.0, 779.1, 781.2, and 797.3). This study sheds light on a new culture system in which the MLV concentration could change the lipid profile of the embryonic cell membrane in a dose-dependent manner Ver menos