S-nitrosothiol-terminated Pluronic F127 : influence of microstructure on nitric oxide release
Guilherme F. Picheth, Laura C. E. da Silva, Leonardo P. Giglio, Tomas S.) Plivelic, Marcelo G. de Oliveira
ARTIGO
Inglês
Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing....
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Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications. Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, H-1 NMR spin-spin relaxation (T-2), temperature-dependent small-angle X-ray scattering, and cryo-transmission electron microscopy, were used to determine the micellar packing structure, while real-time chemiluminescence NO detection and UV-Vis spectrophotometry were used to evaluate the kinetics of NO release. HS-F127-SH micellization and gelation processes were analogous to native F127, however, with a decreased short-range ordering of the micelles. ONS-F127-SNO hydrogels released NO thorough a preferentially intramicellar SNO dimerization reaction. Increasing ONS-F127-SNO concentration reduces the rate of SNO dimerization and increases the overall rate of NO release to the gas phase, opening up new possibilities for tailoring NO delivery from F127-based hydrogels
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2016/02414-5; 2017/19253-7; 2018/14142-5; 2017/04622-7
Fechado
S-nitrosothiol-terminated Pluronic F127 : influence of microstructure on nitric oxide release
Guilherme F. Picheth, Laura C. E. da Silva, Leonardo P. Giglio, Tomas S.) Plivelic, Marcelo G. de Oliveira
S-nitrosothiol-terminated Pluronic F127 : influence of microstructure on nitric oxide release
Guilherme F. Picheth, Laura C. E. da Silva, Leonardo P. Giglio, Tomas S.) Plivelic, Marcelo G. de Oliveira
Fontes
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Journal of colloid and interface science Vol. 576 (Sep., 2020), p. 457-467 |