Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)
André V. Scatigna, Fernanda A. Oliveira, Camila C. Mantello, Patrícia M. Francisco, Anete P. Souza, André O. Simões
ARTIGO
Inglês
Premise of the study: Microsatellite markers were developed for the critically endangered carnivorous species Philcoxia minensis (Plantaginaceae) for further population genetic studies aiming at its conservation. Methods and Results: We identifi ed 29 clones containing 40 microsatellites from a...
Ver mais
Premise of the study: Microsatellite markers were developed for the critically endangered carnivorous species Philcoxia minensis (Plantaginaceae) for further population genetic studies aiming at its conservation. Methods and Results: We identifi ed 29 clones containing 40 microsatellites from a genomic enriched library. A total of 27 primer pairs were developed and evaluated in 30 individuals of a natural P. minensis population. Seventeen markers successfully presented amplifi cation products within the expected size range, of which 12 were polymorphic. The expected and observed heterozygosities ranged from 0.03 to 0.65 and from 0.00 to 0.77, respectively. Positive transferability with the related species P. bahiensis was observed for the same 17 markers. Conclusions: The 12 polymorphic microsatellite markers are suitable for studies in genetic diversity and structure, mating system, and gene fl ow in P. minensis and also may be useful for similar issues regarding the related species P. bahiensis
Ver menos
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2012/12927-9
Aberto
Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)
André V. Scatigna, Fernanda A. Oliveira, Camila C. Mantello, Patrícia M. Francisco, Anete P. Souza, André O. Simões
Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)
André V. Scatigna, Fernanda A. Oliveira, Camila C. Mantello, Patrícia M. Francisco, Anete P. Souza, André O. Simões
Fontes
|
Applications in plant sciences Vol. 3, no. 8 (Aug., 2015), n. art. 1500035 |