Plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promisingalternative for the biological control of pests. In this work, we tested the anti-insect activity ofAdenan-thera pavonina trypsin inhibitor (ApTI) against Diatraea saccharalis larvae, a major...

Plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promisingalternative for the biological control of pests. In this work, we tested the anti-insect activity ofAdenan-thera pavonina trypsin inhibitor (ApTI) against Diatraea saccharalis larvae, a major insect pest in sugar-cane. The addition of 0.1% ApTI in short-term assays resulted in 87% and 63% decreased trypsin andchymotrypsin activities respectively. ApTI was not digested after 60 h incubation withD. saccharalismid-gut proteases. The chronic effects of ApTI on F0 and F1 generations of D. saccharalis were also analyzed.The larvae from the F0 generation showed 55% and 21% decreased larval and pupal viability, respectively.ApTI-fed larvae from the F1 generation showed a decrease of 33% in survival rate and 23% in the averagelarval weight. Moreover, ApTI treatment reduced trypsin and chymotrypsin activities in F1 larvae. Thus,the anti-insect effects of ApTI on consecutive generations (F0 and F1) of D. saccharalis larvae demonstrateits potential for long-term control of this pest.

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

FINANCIADORA DE ESTUDOS E PROJETOS - FINEP

FUNDAÇÃO DE APOIO AO DESENVOLVIMENTO DO ENSINO, CIÊNCIA E TECNOLOGIA DO ESTADO DE MATO GROSSO DO SUL - FUNDECT

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