Changes in patterns of metachromatic staining due to RNA were followed by a critical electrolyte concentration method in culture cells during mitosis, and compared with Ag-NOR staining results and with published data especially referring to nucleolar/nuclear proteins. A deep metachromatic response...

Changes in patterns of metachromatic staining due to RNA were followed by a critical electrolyte concentration method in culture cells during mitosis, and compared with Ag-NOR staining results and with published data especially referring to nucleolar/nuclear proteins. A deep metachromatic response was found in association with the chromosomes, radiating along the spindle structure and then becoming greatly reduced in nuclei at late telophase/early cytokinesis. This response distribution was not exactly coincident with the reported relocation of nucleolar/nuclear proteins or with a non-nucleolar RNA-containing layer during mitosis. The sites of RNA metachromasy also differed from those of Ag-NOR positive response. The results indicate that RNA most probably of nucleolar origin participates in the complex architecture which is assumed to protect the chromosomes, to store rRNA maturation factors, and to contribute to distribution of nucleolar/nuclear proteins and particular RNA types to daughter cells.

Changes in patterns of metachromatic staining due to RNA were followed by a critical electrolyte concentration method in culture cells during mitosis, and compared with Ag-NOR staining results and with published data especially referring to nucleolar/nuclear proteins. A deep metachromatic response...

Changes in patterns of metachromatic staining due to RNA were followed by a critical electrolyte concentration method in culture cells during mitosis, and compared with Ag-NOR staining results and with published data especially referring to nucleolar/nuclear proteins. A deep metachromatic response was found in association with the chromosomes, radiating along the spindle structure and then becoming greatly reduced in nuclei at late telophase/early cytokinesis. This response distribution was not exactly coincident with the reported relocation of nucleolar/nuclear proteins or with a non-nucleolar RNA-containing layer during mitosis. The sites of RNA metachromasy also differed from those of Ag-NOR positive response. The results indicate that RNA most probably of nucleolar origin participates in the complex architecture which is assumed to protect the chromosomes, to store rRNA maturation factors, and to contribute to distribution of nucleolar/nuclear proteins and particular RNA types to daughter cells.

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

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