Agradecimentos: N.M.S. acknowledges the São Paulo Research Foundation (Fapesp, Brazil) (Grant no. 2012/19167-0 and 2015/21546-7), and R.V.R. and E.C.M. acknowledge the fellowship granted by the National Council for Scientific and Technological Development (CNPq, Brazil). The authors also acknowledge...

Agradecimentos: N.M.S. acknowledges the São Paulo Research Foundation (Fapesp, Brazil) (Grant no. 2012/19167-0 and 2015/21546-7), and R.V.R. and E.C.M. acknowledge the fellowship granted by the National Council for Scientific and Technological Development (CNPq, Brazil). The authors also acknowledge the Centre for Research in Biosciences at University of the West of England (UWE)

Abstract: Many assays focus on determining NO content within plant tissues to assess the actual concentration that impacts on cellular processes. Diaminofluorescein fluorescent dyes (DAFs) have been very widely used by plant scientists to reveal likely sites of NO production inside and outside...

Abstract: Many assays focus on determining NO content within plant tissues to assess the actual concentration that impacts on cellular processes. Diaminofluorescein fluorescent dyes (DAFs) have been very widely used by plant scientists to reveal likely sites of NO production inside and outside cells. In general, DAFs dyes react with N2O3, a byproduct of NO oxidation, resulting in fluorescence. It is initially available in the form of diacetate (DAF-2DA), which allowed the ready absorption by the cells. The diacetate group is removed by cell esterases leaving the membrane impermeable to DAF-2 and available for N2O3 nitration to generate the highly fluorescent triazole (DAF-2T). Here, we describe two methods for detection of NO by fluorescence, one for NO extracellular detection by DAF-2 and the other one for NO intracellular detection, in this case using DAF-2DA

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

2012/19167-0; 2015/21546-7

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

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