Association of TNF polymorphisms with JAK2 (V617F) myeloproliferative neoplasms in brazilian patients
Luciana Conci Macedo, Fernanda de Cesare Quintero, Sara Pagliari-E-Silva, Katia Borgia Barbosa Pagnano, Camila Rodrigues, Josiane Bazzo de Alencar, Ana Maria Sell, Jeane Eliete Laguila Visentainer
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Inglês
The classical chromosome Philadelphia-negative myeloproliferative neoplasms (MPNs) are a group of disorders that share clinical, hematological, and histological features. Proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) are elevated in patients with MPN. The aim of this study was to...
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The classical chromosome Philadelphia-negative myeloproliferative neoplasms (MPNs) are a group of disorders that share clinical, hematological, and histological features. Proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) are elevated in patients with MPN. The aim of this study was to verify the association between the polymorphisms of TNF gene (-308G/A and -238 G/A) in BCR-ABL-negative MPN in our population. Blood samples obtained from MPN patients were genotyped for the JAK2V617F mutation and both TNF polymorphisms using PCR-RFLP. Thirty three (26.8%) patients with polycythemia vera (PV), 35 (28.7%) essential thrombocythemia (ET), 22 (17.7%) primary myelofibrosis (PMF), and 33 (26.8%) with unclassifiable MPN (MPNu) were included in the study. The JAK2 V617F mutation was detected in 94 (76.42%) patients. Were observed a significant increase on the frequency of the TNF-238 GA genotype in MPN patients compared to controls (OR = 2.21, 95% CI = 1.02–4.80, P < 0.04). The distribution of the genotypes and allelic frequencies of TNF-308 was significantly different among the MPNs, JAK2V617F positive, PV and PMF, and controls. Our data has demonstrated that the polymorphisms on TNF-238 GA, TNF-308 GA were associated to MPN development in this population, triggered by JAK2 V617F mutation
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Association of TNF polymorphisms with JAK2 (V617F) myeloproliferative neoplasms in brazilian patients
Luciana Conci Macedo, Fernanda de Cesare Quintero, Sara Pagliari-E-Silva, Katia Borgia Barbosa Pagnano, Camila Rodrigues, Josiane Bazzo de Alencar, Ana Maria Sell, Jeane Eliete Laguila Visentainer
Association of TNF polymorphisms with JAK2 (V617F) myeloproliferative neoplasms in brazilian patients
Luciana Conci Macedo, Fernanda de Cesare Quintero, Sara Pagliari-E-Silva, Katia Borgia Barbosa Pagnano, Camila Rodrigues, Josiane Bazzo de Alencar, Ana Maria Sell, Jeane Eliete Laguila Visentainer
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Blood cells, molecules and diseases Vol. 57 (Mar., 2016), p. 54-57 |