Exosomes in the serum of acute myeloid leukemia patients induce dendritic cell tolerance : implications for immunotherapy
Bruno Deltreggia Benites, Adriana da Silva Santos Duarte, Ana Leda Figueiredo Longhini, Irene Santos, Marisa Claudia Alvarez, Ligia Nunes de Morais Ribeiro, Eneida de Paula, Sara Teresinha Olalla Saad
ARTIGO
Inglês
Exosomes may represent an interesting antigenic pulse for new forms of anti-tumor immunotherapy. We evaluated exosomes from serum of patients with acute myeloid leukemia (AML) as an antigenic source for dendritic cells (DC) and the effects upon antitumor cytotoxicity, assessed by the percentage of...
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Exosomes may represent an interesting antigenic pulse for new forms of anti-tumor immunotherapy. We evaluated exosomes from serum of patients with acute myeloid leukemia (AML) as an antigenic source for dendritic cells (DC) and the effects upon antitumor cytotoxicity, assessed by the percentage of specific lysis of K562 leukemic cells in co-cultures. Surprisingly, incubation of exosomes with DCs decreased lysis of K562, which may correspond to a mechanism of tumor evasion in vivo. However, when immature DCs were pulsed with exosomes purified from K562 culture supernatants, the lysis of target cells was notably enhanced, associated with a substantial increase in the expression of the maturation marker CD83. Thus, the development of vaccines using patients' exosomes would probably add no benefits to the treatment of AML; alternately, exosomes from cultured cells may represent an effective way for maturing DCs into a cytotoxic phenotype, without the immunosuppression observed with patients' exosomes. (C) 2019 Elsevier Ltd. All rights reserved
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2011/51959-0
fechado
Exosomes in the serum of acute myeloid leukemia patients induce dendritic cell tolerance : implications for immunotherapy
Bruno Deltreggia Benites, Adriana da Silva Santos Duarte, Ana Leda Figueiredo Longhini, Irene Santos, Marisa Claudia Alvarez, Ligia Nunes de Morais Ribeiro, Eneida de Paula, Sara Teresinha Olalla Saad
Exosomes in the serum of acute myeloid leukemia patients induce dendritic cell tolerance : implications for immunotherapy
Bruno Deltreggia Benites, Adriana da Silva Santos Duarte, Ana Leda Figueiredo Longhini, Irene Santos, Marisa Claudia Alvarez, Ligia Nunes de Morais Ribeiro, Eneida de Paula, Sara Teresinha Olalla Saad
Fontes
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Vaccine Vol. 37, no. 11 (Mar., 2019), p. 1377-1383 |