Post‐translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
ARTIGO
Inglês
ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa‐ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells....
ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa‐ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co‐immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co‐localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
101624/2011‐5
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
09/07322‐8; 07/54870‐5; 07/08019‐1
Fechado
Post‐translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
Post‐translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
Fontes
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FEBS letters Vol. 586, no. 19 (2012), p. 3522-3528 |