Agradecimentos: This study was founded by "Fundação de Amparo à Pesquisa do Estado de São Paulo" (FAPESP Process: 2014/08829-7), "Conselho Nacional de Desenvolvimento Científico e Tecnológico" (CNPq-PVE Process: 400362/2014-7), "Coordenação de Aperfeiçoamento de Pessoal de Nível Superior" (CAPES),...

Agradecimentos: This study was founded by "Fundação de Amparo à Pesquisa do Estado de São Paulo" (FAPESP Process: 2014/08829-7), "Conselho Nacional de Desenvolvimento Científico e Tecnológico" (CNPq-PVE Process: 400362/2014-7), "Coordenação de Aperfeiçoamento de Pessoal de Nível Superior" (CAPES), "Instituto Nacional de Ciência, Tecnologia e Informação em Materiais Complexos Funcionais" (INOMAT). The authors thank M. Flynn, A. Caloto-Oliveira and G. Almeida for the organism collection, F. Leite and S.L. Gomes for the organism identification

Abstract: There is a lack of suitable tropical marine species for ecotoxicity tests. An attractive model organism for ecotoxicology is the marine amphipod Parhyale hawaiensis, which is already a model for genetic and developmental studies. This species is widespread, can tolerate changes in...

Abstract: There is a lack of suitable tropical marine species for ecotoxicity tests. An attractive model organism for ecotoxicology is the marine amphipod Parhyale hawaiensis, which is already a model for genetic and developmental studies. This species is widespread, can tolerate changes in salinity, is easy to handle and is representative of circumtropical regions. The aim of this work was to describe standardized procedures for laboratory husbandry, define conditions for acute toxicity tests, and to provide acute toxicity test results for some reference toxicants. Culturing conditions for the organism in the laboratory were established in reconstituted seawater (30 ± 2 salinity), 24 ± 2 °C, photoperiod 12/12 h light/dark. Acute toxicity test procedures were developed for 96 h-exposure time, and organisms at ages <7 days. The miniaturized version of the test, based on 96-well microplates and 200 µL of exposure media provided consistent results compared to larger exposure volumes (80-mL vials protocol). Acute toxicity of Ag, Cd, Cu, Zn and ammonia determined for P. hawaiensis were consistent to previous results for other marine amphipods. We conclude that P. hawaiensis can be successfully cultured in standardized conditions and be effectively used in acute toxicity testing. Further development and use of this model will enable standardized and reproducible ecotoxicology investigations in understudied and vulnerable tropical marine ecosystems

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

400362/2014-7

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

2014/08829-7

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

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