Agradecimentos: A.C. de Camargo acknowledges Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Process 88887.169471/2018-00) and Fondo Nacional de Desarrollo Científico y Tecnológico – FONDECYT postdoctorado (project 3180432). A. R. Schwember is thankful to the Fundación para la...

Agradecimentos: A.C. de Camargo acknowledges Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Process 88887.169471/2018-00) and Fondo Nacional de Desarrollo Científico y Tecnológico – FONDECYT postdoctorado (project 3180432). A. R. Schwember is thankful to the Fundación para la Innovación Agraria - FIA (Project PYT-2017-0490). D. Granato acknowledges Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq for a productivity grant (Process 303188/2016-2). M. Franchin acknowledges FAPESP (Process 2016/15563-9). P. L. Rosalen is thankful to CNPq (Process 310522/2015-3). S. M. Alencar acknowledges FAPESP (Process 2008/55492-7). F. Shahidi thanks the Natural Science and Engineering Research Council (NSERC) of Canada for a Discovery Grant

Free radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their...

Free radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their ability in anticipating the reduction of the activation of NF-kappa 3 using LPS-activated RAW 264.7 macrophages, selected as a biological model. Grape processing by-products from winemaking showed higher total phenolic content (TPC), antioxidant capacity towards peroxyl radical (31.1%) as well as reducing power (39.5%) than those of grape juice by-products. The same trend was observed when these samples were tested against LPS-activated RAW 264.7 macrophages by reducing the activation NF-kappa B. Feedstocks containing higher TPC and corresponding ORAC and FRAP results translated to higher reduction in the activation of NF-kappa 3 (36.5%). Therefore, this contribution demonstrates that colorimetric methods are still important screening tools owing their simplicity and widespread application

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

303188/2016-2; 310522/2015-3

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

88887.169471/2018-00

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

2008/55492-7; 2016/15563-9

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