15d-PGJ(2)-loaded solid lipid nanoparticles : physicochemical characterization and evaluation of pharmacological effects on inflammation
Nathalie Ferreira Silva de Melo, Cristina Gomes de Macedo, Ricardo Bonfante, Henrique Ballassini Abdalla, Camila Morais Goncalves da Silva, Tatiane Pasquoto, Renata de Lima, Leonardo Fernandes Fraceto, Juliana Trindade Clemente-Napimoga, Marcelo Henrique Napimoga
ARTIGO
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15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), a peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist, has physiological properties including pronounced anti-inflammatory activity, though it binds strongly to serum albumin. The use of solid lipid nanoparticles (SLN) can...
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15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), a peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist, has physiological properties including pronounced anti-inflammatory activity, though it binds strongly to serum albumin. The use of solid lipid nanoparticles (SLN) can improve therapeutic properties increasing drug efficiency and availability. 15d-PGJ(2)-SLN was therefore developed and investigated in terms of its immunomodulatory potential. 15d-PGJ(2)-SLN and unloaded SLN were physicochemically characterized and experiments in vivo were performed. Animals were pretreated with 15d-PGJ(2)-SLN at concentrations of 3, 10 or 30 mu g.kg(-1) before inflammatory stimulus with carrageenan (Cg), lipopolysaccharide (LPS) or mBSA (immune response). Interleukins (IL-1 beta, IL-10 and IL-17) levels were also evaluated in exudates. The 15d-PGJ(2)-SLN system showed good colloidal parameters and encapsulation efficiency of 96%. The results showed that the formulation was stable for up to 120 days with low hemolytic effects. The 15d-PGJ(2)-SLN formulation was able to reduce neutrophil migration in three inflammation models tested using low concentrations of 15d-PGJ(2). Additionally, 15d-PGJ(2)-SLN increased IL-10 levels and reduced IL-1 beta as well as IL-17 in peritoneal fluid. The new 15d-PGJ(2)-SLN formulation highlights perspectives of a potent anti-inflammatory system using low concentrations of 15d-PGJ(2)
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
303555/2013-0
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2014/11016-8
aberto
15d-PGJ(2)-loaded solid lipid nanoparticles : physicochemical characterization and evaluation of pharmacological effects on inflammation
Nathalie Ferreira Silva de Melo, Cristina Gomes de Macedo, Ricardo Bonfante, Henrique Ballassini Abdalla, Camila Morais Goncalves da Silva, Tatiane Pasquoto, Renata de Lima, Leonardo Fernandes Fraceto, Juliana Trindade Clemente-Napimoga, Marcelo Henrique Napimoga
15d-PGJ(2)-loaded solid lipid nanoparticles : physicochemical characterization and evaluation of pharmacological effects on inflammation
Nathalie Ferreira Silva de Melo, Cristina Gomes de Macedo, Ricardo Bonfante, Henrique Ballassini Abdalla, Camila Morais Goncalves da Silva, Tatiane Pasquoto, Renata de Lima, Leonardo Fernandes Fraceto, Juliana Trindade Clemente-Napimoga, Marcelo Henrique Napimoga
Fontes
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PLoS one Vol. 11, no. 8 (Aug., 2016), p. e0161796 |