Effects of 7-epiclusianone on Streptococcus mutans and caries development in rats
Luciana Salles Branco-de-Almeida, Ramiro Mendonça Murata, Eliane Melo Franco, Marcelo Henrique dos Santos, Severino Matias de Alencar, Hyun Koo, Pedro Luiz Rosalen
ARTIGO
Inglês
The aim of this study was to evaluate the effects of 7-epiclusianone (7-epi) on specific virulence attributes of Streptococcus mutans in vitro and on development of dental caries in vivo. 7-Epi was obtained and purified from fruits of Rheedia brasiliensis. We investigated its influence on...
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The aim of this study was to evaluate the effects of 7-epiclusianone (7-epi) on specific virulence attributes of Streptococcus mutans in vitro and on development of dental caries in vivo. 7-Epi was obtained and purified from fruits of Rheedia brasiliensis. We investigated its influence on surface-adsorbed glucosyltransferase (Gtf) B activity, acid production, and viability of S. mutans in biofilms, as well as on caries development using a rodent model. 7-Epi (100 mu g/mL) significantly reduced the activity of surface-adsorbed GtfB (up to 48.0 +/- 1.8 of inhibition at 100 mu g/mL) and glyco-lytic pH-drop by S. mutans in biofilms (125 and 250 mu g/mL) (vs. vehicle control, p < 0.05). In contrast, the test compound did not significantly affect the bacterial viability when compared to vehicle control (15% ethanol, p > 0.05). Wistar rats treated topically with 7-epi (twice daily, 60-s exposure) showed significantly smaller number of and less severe smooth-and sulcal-surface carious lesions (p < 0.05), without reducing the S. mutans viable population from the animals` dental biofilms. In conclusion, the natural compound 7-epiclusianone may be a potentially novel pharmacological agent to prevent and control dental caries disease
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
131751/2006-9; 141253/2005-3
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2006/56379-4
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
fechado
Effects of 7-epiclusianone on Streptococcus mutans and caries development in rats
Luciana Salles Branco-de-Almeida, Ramiro Mendonça Murata, Eliane Melo Franco, Marcelo Henrique dos Santos, Severino Matias de Alencar, Hyun Koo, Pedro Luiz Rosalen
Effects of 7-epiclusianone on Streptococcus mutans and caries development in rats
Luciana Salles Branco-de-Almeida, Ramiro Mendonça Murata, Eliane Melo Franco, Marcelo Henrique dos Santos, Severino Matias de Alencar, Hyun Koo, Pedro Luiz Rosalen
Fontes
Planta medica: journal of medicinal plant and natural product research (Fonte avulsa) |