Tissue microarray use for immunohistochemical study of ameloblastoma
Rodrigo Neves-Silva, Felipe Paiva Fonseca, Adriana Souza de Jesus, Hélder Antônio Rebelo Pontes, André Caroli Rocha, Thais Bianca Brandão, Pablo Agustin Vargas, Márcio Ajudarte Lopes, Alan Roger Santos-Silva, Oslei Paes de Almeida
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Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms,...
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Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. Forty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). Kappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). Using a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS
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Tissue microarray use for immunohistochemical study of ameloblastoma
Rodrigo Neves-Silva, Felipe Paiva Fonseca, Adriana Souza de Jesus, Hélder Antônio Rebelo Pontes, André Caroli Rocha, Thais Bianca Brandão, Pablo Agustin Vargas, Márcio Ajudarte Lopes, Alan Roger Santos-Silva, Oslei Paes de Almeida
Tissue microarray use for immunohistochemical study of ameloblastoma
Rodrigo Neves-Silva, Felipe Paiva Fonseca, Adriana Souza de Jesus, Hélder Antônio Rebelo Pontes, André Caroli Rocha, Thais Bianca Brandão, Pablo Agustin Vargas, Márcio Ajudarte Lopes, Alan Roger Santos-Silva, Oslei Paes de Almeida
Fontes
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Journal of oral pathology and medicine Vol. 45, no. 9 (Oct., 2016), p. 704-711 |