Dependence of cytokine levels on the sex of experimental animals : a pilot study on the effect of oestrogen in the temporomandibular joint synovial tissues
S. R. Figueroba, G. C. N. Franco, N. F. Omar, M. F. Groppo, F. C. Groppo
ARTIGO
Inglês
The aim of this study was to evaluate the effects of 17β-oestradiol (E2) on cartilage thickness and cytokine levels in the temporomandibularjoint (TMJ). Thirty rats (15 female, 15 male) were orchidectomized (ORX), ovariectomized (OVX), or sham-operated. After 21 days, animalswere assigned to six...
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The aim of this study was to evaluate the effects of 17β-oestradiol (E2) on cartilage thickness and cytokine levels in the temporomandibularjoint (TMJ). Thirty rats (15 female, 15 male) were orchidectomized (ORX), ovariectomized (OVX), or sham-operated. After 21 days, animalswere assigned to six groups: (1) sham-ORX; (2) ORX; (3) ORX+E2; (4) sham-OVX; (5) OVX; and (6) OVX+E2. Treatments were administered daily for 21 days. The thickness of cartilage layers (fibrous, proliferative, maturation, and hypertrophic) and cytokine levels(interleukins IL-1α, IL-1β, IL-6, and tumour necrosis factor alpha (TNF-α)) were measured by histomorphometry and ELISA, respectively. Kruskal-Wallis/Dunn's tests were used (alpha=5%). Sham-ORX showed thicker layers than ORX+E2, but not thicker than ORX. All layers, except the hypertrophic layer, were thicker in sham-OVX than OVX or OVX+E2. Although IL-1β levels were higher in castrated animals, E2 did not affect the level of this cytokine. IL-1α levels were higher in both ORX (P=0.0010) and ORX+E2 (P=0.0053) than in sham-ORX. However, E2 decreased IL-1α levels in OVX (P=0.0129). When compared to sham-ORX/OVX, IL-6 levels were not affected by E2 in males but were reduced in OVX (P=0.0079) and increased in OVX+E2 (P=0.0434). Levels of TNF-α were reduced by E2 in both ORX+E2 and OVX+E2. E2 treatment caused gender- and layer-dependent changes in the cartilage. Castration increased all cytokine levels, except for IL-6, without respect to gender
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2006/05023-5
fechado
Dependence of cytokine levels on the sex of experimental animals : a pilot study on the effect of oestrogen in the temporomandibular joint synovial tissues
S. R. Figueroba, G. C. N. Franco, N. F. Omar, M. F. Groppo, F. C. Groppo
Dependence of cytokine levels on the sex of experimental animals : a pilot study on the effect of oestrogen in the temporomandibular joint synovial tissues
S. R. Figueroba, G. C. N. Franco, N. F. Omar, M. F. Groppo, F. C. Groppo
Fontes
International journal of oral and maxillofacial surgery (Fonte avulsa) |