Agradecimentos: This study received financial support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior [PROCAD-251/2007 and Edital CAPES/EMBRAPA n° 15/2014 (Proposal 158)], and FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul – Edital PqG - Grant number #2162-2551/13-5). Ver menos

Abstract: This study evaluated the biocompatibility and antibiofilm effects on Streptococcus mutans of an experimental self-etching adhesive system formulated with a natural vegetable product. The cytotoxicity of Clearfil Protect Bond (CPB), Clearfil SE Bond (CSEB), Adper SE Plus (AP), an experimental adhesive containing Butia capitata oil (EA) and an oil-free experimental adhesive as control (CA) was tested on 3T3/NIH mouse fibroblasts. Genotoxicity was indicated by micronuclei formation, and cell alterations were analyzed using light microscopy. To evaluate the effect of the adhesives on S. mutans biofilm, biomass samples were collected for dry weight and bacterial viability analyses, and the pH of the culture media was determined daily as an indicator of biofilm acidogenicity. The CA primer and EA uncured bond adhesive were the least toxic. No statistical difference was observed between EA and the untreated control, and EA showed similar effect to CPB. These findings suggest EA is biocompatible and presents activity against S. mutans biofilm. Ver menos