Agradecimentos: The authors thank Izilvânia Barros (Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil) for her expertise in conducting the microbiologic analysis. The authors also thank Milla Sprone Tavares (Laboratory Assistant, Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil) for her support in PCR analyses and Marina Del Arco (Laboratory Assistant, Department of Clinical Analyses, School of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo) for her technical support during the microbiologic procedures. The study was supported by the São Paulo Research Foundation (FAPESP – process #2013/25022-7), awarded to Michel R. Messora. The authors report no conflicts of interest related to this study. Ver menos

Abstract: Background: This study evaluates effects of topical administration of probiotic bacteria of the genus Bifidobacterium on experimental periodontitis (EP) in rats.
Methods: Thirty-two rats were divided into groups C (control; without EP), EP (EP only), C-HN019 (control+probiotic), and EP-HN019 (EP+probiotic). On day 0 of the experiment, animals of groups EP and EP-HN019 received cotton ligatures around mandibular first molars (MFMs). In groups C-HN019 and EP-HN019, 1 mL of suspensions containing Bifidobacterium animalis subsp. lactis (B. lactis) HN019 was topically administered in the subgingival region of MFMs on days 0, 3, and 7. In groups C and EP, topical administrations were performed using a sham suspension (without probiotic). All animals were euthanized at day 14. Gingival tissue, hemimandibles, and oral biofilm were collected. Data were statistically analyzed (P < 0.05).
Results: Group EP presented greater bone porosity, trabecular separation, and connective tissue attachment loss (CTAL) as well as reduced bone volume than all other groups (P < 0.05). In group EP-HN019, there were greater proportions of Actinomyces and Streptococcus-like species and lower proportions of Veillonella parvula, Capnocytophaga sputigena, Eikenella corrodens, and Prevotella intermedia-like species than group EP. Group EP-HN019 presented greater expressions of osteoprotegerin and beta-defensins than group EP (P < 0.05). Group EP presented greater levels of interleukin-1 beta and receptor activator of nuclear factor-kappa B ligand than group EP-HN019 (P < 0.05).
Conclusion: Topical use of B. lactis HN019 promotes a protective effect against alveolar bone loss and CTAL sattributable to EP in rats, modifying immunoinflammatory and microbiologic parameters Ver menos