Leucine‐rich amelogenin peptide (lrap) uptake by cementoblast requires Flotillin‐1 mediated endocytosis
Luciane Martins, Adriana Franco Paes Leme, Kamila Rosamilia Kantovitz, Em Nome de Luciane Martins, Enilson Antonio Sallum, Marcio Zaffalon Casati, Jr. Nociti Francisco Humberto
ARTIGO
Inglês
Agradecimentos: This research was supported by National Council for Scientific and Technological Development (CNPq, grant # 479692/2012-2) and Coordination for the Improvement of the Higher Level Personnel (CAPES, grant # 02426/09-9 and 33003033008P8). The authors thank Dr. Carolyn W. Gibson and Dr....
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Agradecimentos: This research was supported by National Council for Scientific and Technological Development (CNPq, grant # 479692/2012-2) and Coordination for the Improvement of the Higher Level Personnel (CAPES, grant # 02426/09-9 and 33003033008P8). The authors thank Dr. Carolyn W. Gibson and Dr. Martha Somerman for kindly providing the bacterial expression vector p56 and the cementoblast-like cell line OCCM-30, respectively.
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Abstract: Basic, pre-clinical, and clinical studies have documented the potential of amelogenin, and its variants, to affect cell response and tissue regeneration. However, the mechanisms are unclear. Thus, the aim of the present study was to identify, in cementoblasts, novel binding partners for an...
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Abstract: Basic, pre-clinical, and clinical studies have documented the potential of amelogenin, and its variants, to affect cell response and tissue regeneration. However, the mechanisms are unclear. Thus, the aim of the present study was to identify, in cementoblasts, novel binding partners for an alternatively spliced amelogenin form (Leucine-Rich Amelogenin PeptideLRAP), which is supposed to act as a signaling molecule in epithelial-mesenchymal interactions. LRAP-binding protein complexes from immortalized murine cementoblasts (OCCM-30) were achieved by capture affinity assay (GST pull down) and proteins present in these complexes were identified by mass spectrometry and immunoblotting. Flotillin-1, which functions as a platform for signal transduction, vesicle trafficking, endocytosis, and exocytosis, was identified and confirmed by co-precipitation and co-localization assays as a protein-binding partner for LRAP in OCCM-30 cells. In addition, we found that exogenously added GST-LRAP recombinant protein was internalized by OCCM-30 cells, predominantly localized in the perinuclear region and, that inhibition of flotillin1-dependent functions by small interference RNA (siRNA) methodology significantly affected LRAP uptake and its biological properties on OCCM-30 cells, including LRAP effect on the expression of genes encoding osteocalcin (Ocn), bone sialoprotein (Bsp), and runt-related transcription factor 2 (RunX2). In conclusion, LRAP uptake by cementoblast involves flotillin-assisted endocytosis, which suggests an involvement of LRAP in lipid-raft-dependent signaling pathways which are mediated by flotillin-1
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COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
02426/09-9; 33003033008P8
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
479692/2012-2
Fechado
Leucine‐rich amelogenin peptide (lrap) uptake by cementoblast requires Flotillin‐1 mediated endocytosis
Luciane Martins, Adriana Franco Paes Leme, Kamila Rosamilia Kantovitz, Em Nome de Luciane Martins, Enilson Antonio Sallum, Marcio Zaffalon Casati, Jr. Nociti Francisco Humberto
Leucine‐rich amelogenin peptide (lrap) uptake by cementoblast requires Flotillin‐1 mediated endocytosis
Luciane Martins, Adriana Franco Paes Leme, Kamila Rosamilia Kantovitz, Em Nome de Luciane Martins, Enilson Antonio Sallum, Marcio Zaffalon Casati, Jr. Nociti Francisco Humberto
Fontes
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Journal of cellular physiology Vol. 232, no. 3 (Mar., 2017), p. 556-565 |