Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents
ARTIGO
Inglês
Agradecimentos: This research was supported by Brazilian Financial Agencies: CNPq: 140698/2013-2 and 310522/2015-3, FAPESP: 2013/22823-9 and 2014/17543-0. The authors are indebted to Mr. R. Salaroli and Dr. E. W. Kitajima (NAP/MEPA-ESALQ/USP) for technical support in scanning electron microscopy....
Agradecimentos: This research was supported by Brazilian Financial Agencies: CNPq: 140698/2013-2 and 310522/2015-3, FAPESP: 2013/22823-9 and 2014/17543-0. The authors are indebted to Mr. R. Salaroli and Dr. E. W. Kitajima (NAP/MEPA-ESALQ/USP) for technical support in scanning electron microscopy. The composition of restorative materials containing Apigenin and tt-Farnesol is covered by the patent: Dental restorative composition and its use (BR 10 2014 0244975)
Abstract: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Apigenin (1 mM) and tt-Farnesol (5 mM) were added separately and in...
Abstract: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Apigenin (1 mM) and tt-Farnesol (5 mM) were added separately and in combination to a self-etch adhesive (CS3 — Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT — OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115 h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. Addition of Apigenin and Apigenin + tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin + tt-Farnesol to CS3. No changes in dentin bond strength, resin–dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
140698/2013-2; 310522/2015-3
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2013/22823-9; 2014/17543-0
Fechado
Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents
Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents
Fontes
Dental materials Vol. 33, no. 10 (Oct., 2017), p. 1084-1092 |