Immune-inflammatory cell profile and receptor activator of nuclear factor kappa B ligand/osteoprotegerin expression in persistent apical periodontitis after root canal retreatment failure
Carlos Estrela, Daniel de Almeida Decurcio, Júlio Almeida Silva, Aline Carvalho Batista, Nathália Caroline de Souza Lima, Brunno Santos de Freitas Silva, João Antonio Chaves de Souza, Carlos Alberto Souza Costa
ARTIGO
Inglês
This study assessed the immune-inflammatory profile and the expression of bone resorption activators receptor activator of nuclear factor kappa B ligand (RANKL) and inhibitor osteoprotegerin (OPG) in apical periodontitis (n = 20) that persisted after root canal retreatment. Methods...
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This study assessed the immune-inflammatory profile and the expression of bone resorption activators receptor activator of nuclear factor kappa B ligand (RANKL) and inhibitor osteoprotegerin (OPG) in apical periodontitis (n = 20) that persisted after root canal retreatment. Methods Immunohistochemistry was used to characterize lymphocyte populations (CD3+, CD45RO+, CD8+, and FoxP3+ cells), macrophages (CD68+), RANKL+ and OPG+ cells in persistent apical periodontitis (PAP) and primary periapical lesions (PPLs). By using quantitative real-time polymerase chain reaction, the mRNA expression of RANKL and OPG in PAP and periodontal ligament from healthy teeth was comparatively analyzed. The data were analyzed by Mann-Whitney, Pearson χ2, and Wilcoxon tests (5% level). Results PAP showed an elevated number of FoxP3+ cells compared with PPL (P < .001). The number of CD68+ cells was reduced in the PAP samples compared with the PPLs (P < .001). Similar number of other lymphocyte populations was observed in PAP and PPLs (P > .05 for all comparisons). No differences in the RANKL, OPG, and immune-inflammatory cells were demonstrated when comparing PAP microscopically classified as cyst with those classified as granulomas (P > .05 for all comparisons). The assessment of mRNA expression revealed higher levels of RANKL and OPG in PAP compared with the periodontal ligament from healthy teeth (control) samples (P < .001). Also, a greater expression of RANKL in comparison with OPG was observed in PAP (P < .001). Conclusions These findings indicate that PAP consists of biologically active lesions that demonstrate potential of bone resorption (higher expression of RANKL) and is characterized by an immune-inflammatory cell profile that suggests a suppressive and regulatory environment (higher number of FoxP3+ cells and lower number of macrophages) favorable to more chronic clinical behavior
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
474642/2009-7; 306394/2011-1
Fechado
Immune-inflammatory cell profile and receptor activator of nuclear factor kappa B ligand/osteoprotegerin expression in persistent apical periodontitis after root canal retreatment failure
Carlos Estrela, Daniel de Almeida Decurcio, Júlio Almeida Silva, Aline Carvalho Batista, Nathália Caroline de Souza Lima, Brunno Santos de Freitas Silva, João Antonio Chaves de Souza, Carlos Alberto Souza Costa
Immune-inflammatory cell profile and receptor activator of nuclear factor kappa B ligand/osteoprotegerin expression in persistent apical periodontitis after root canal retreatment failure
Carlos Estrela, Daniel de Almeida Decurcio, Júlio Almeida Silva, Aline Carvalho Batista, Nathália Caroline de Souza Lima, Brunno Santos de Freitas Silva, João Antonio Chaves de Souza, Carlos Alberto Souza Costa
Fontes
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Journal of endodontics Vol. 42, no. 3 (Mar., 2016), p. 439-446 |