This study aimed to investigate whether combined exposure to starch and sucrose modifies the activity of carbonic anhydrase VI (CA VI) in saliva (Study 1) and biofilm (Study 2) of children with early childhood caries (ECC). For Study 1 and Study 2, respectively, 54 and 46 preschoolers aged 4 to 5...

This study aimed to investigate whether combined exposure to starch and sucrose modifies the activity of carbonic anhydrase VI (CA VI) in saliva (Study 1) and biofilm (Study 2) of children with early childhood caries (ECC). For Study 1 and Study 2, respectively, 54 and 46 preschoolers aged 4 to 5 were allocated into two groups: caries-free (CF) and with ECC. Children were exposed to rinses with sucrose, starch, and sucrose plus starch solutions. CA VI activity, pH, and buffering capacity (BC) were evaluated in saliva and biofilm. In Study 1, a significant reduction in saliva pH was observed after sucrose and sucrose plus starch rinses. CA VI activity was influenced by ECC independently of the type of carbohydrate to which children were exposed. CA VI activity was higher in children with ECC; however, after rinses, CA VI activity was reduced. In Study 2, biofilm pH and BC were reduced after rinses with sucrose and sucrose plus starch. CA VI activity was significantly high before rinse in ECC group when compared with CF group; however, no difference was observed between groups after rinses. In saliva, exposure to starch and sucrose (isolated or combined) induced a reduction in CA VI activity in children with ECC. In biofilm, the combination of starch and sucrose did not modify CA VI activity in ECC children. The responsivity of the CA VI reflects directly in important parameters related to the pH maintenance on the oral cavity

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

2017/17630-8

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