Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma
A. L. C. A. Rangel, F. P. Fonseca, O. P. de Almeida
ARTIGO
Inglês
To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis...
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To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis against cytokeratin 7, Ki67, p63, and CD34 were performed and subsequently evaluated with PixelCount, nuclear, and microvessel software applications. The 1.0-mm TMA presented lower results than 2.0-and 3.0-mm TMAs versus conventional whole section slides. Possibly because of an increased amount of stromal tissue, 3.0-mm cores presented a higher microvessel density. Comparing the results obtained with one, two, and three 2.0-mm cores, there was no difference between triplicate or duplicate TMAs and a single-core TMA. Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2009/53839-2; 2012/07519-9; 2012/10781-7
Aberto
Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma
A. L. C. A. Rangel, F. P. Fonseca, O. P. de Almeida
Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma
A. L. C. A. Rangel, F. P. Fonseca, O. P. de Almeida
Fontes
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Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology Vol. 117, no. 1 (Jan., 2014), p. 81-88 |