Transdentinal protective role of sodium ascorbate against the cytopathic effects of H(2)O(2) released from bleaching agents
Adriano Fonseca Lima, Fernanda Campos Rosetti Lessa, Maria Nadir Gasparoto Mancini, Josimeri Hebling, Carlos Alberto de Souza Costa, Giselle Maria Marchi
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The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. Study design: Human dentin discs of 0.5-mm thickness were obtained...
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The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. Study design: Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay. Results: In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells. Conclusions: The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP.
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Transdentinal protective role of sodium ascorbate against the cytopathic effects of H(2)O(2) released from bleaching agents
Adriano Fonseca Lima, Fernanda Campos Rosetti Lessa, Maria Nadir Gasparoto Mancini, Josimeri Hebling, Carlos Alberto de Souza Costa, Giselle Maria Marchi
Transdentinal protective role of sodium ascorbate against the cytopathic effects of H(2)O(2) released from bleaching agents
Adriano Fonseca Lima, Fernanda Campos Rosetti Lessa, Maria Nadir Gasparoto Mancini, Josimeri Hebling, Carlos Alberto de Souza Costa, Giselle Maria Marchi
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Oral surgery, oral medicine, oral pathology, oral radiology and endodontics Vol. 104, no. 4 (Apr., 2010), p. e70-e76 |