Acidulated phosphate fluoride application changes the protein composition of human acquired enamel pellicle
ARTIGO
Inglês
We evaluated, by proteomic analysis, whether the chemical changes provoked on enamel by acidulated phosphate fluoride (APF) application alter the protein composition of acquired enamel pellicle. Enamel slabs, pretreated with distilled water (negative control), phosphoric acid (active control) or APF...
We evaluated, by proteomic analysis, whether the chemical changes provoked on enamel by acidulated phosphate fluoride (APF) application alter the protein composition of acquired enamel pellicle. Enamel slabs, pretreated with distilled water (negative control), phosphoric acid (active control) or APF solution, were immersed in human saliva for pellicle formation. The adsorbed proteins were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Fifty-six proteins were identified, 12 exclusive to APF and 11 to phosphoric acid. APF decreased the concentration of histatin-1, but increased the concentration of S100-A9, which is confirmed by immunoblotting. The findings suggest that APF application changes the acquired enamel pellicle composition
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2009/12368-7; 2010/19278-0
fechado
DOI: https://doi.org/10.1159/000346280
Texto completo: https://www.karger.com/Article/FullText/346280
Acidulated phosphate fluoride application changes the protein composition of human acquired enamel pellicle
Acidulated phosphate fluoride application changes the protein composition of human acquired enamel pellicle
Fontes
Caries research Vol. 47, no. 3 (Apr., 2013), p. 251-258 |