Although the cariostatic effects of CO2 laser on enamel have been shown, its effects on root surface demineralization remains uncertain. The objectives of this in vitro research was to establish safe parameters for a pulsed 10.6 μm CO2 laser and to evaluate its effect on morphological features of...

Although the cariostatic effects of CO2 laser on enamel have been shown, its effects on root surface demineralization remains uncertain. The objectives of this in vitro research was to establish safe parameters for a pulsed 10.6 μm CO2 laser and to evaluate its effect on morphological features of the root surface, as well as on the reduction of root demineralization. Ninety-five human root surfaces were randomly divided into five groups: G1-No treatment (control); G2—2.5 J/cm2; G3—4.0 J/cm2; G4—5.0 J/cm2; and G5—6.0 J/cm2. Intrapulpal temperature was evaluated during root surface irradiation by a thermocouple and morphological changes were evaluated by SEM. After the surface treatment, the specimens were submitted to a 7-day pH-cycling model. Subsequently, the cross-sectional Knoop microhardness values were measured. For all irradiated groups, intrapulpal temperature changes were less than 1.5°C. Scanning electron microscopy images indicated that fluences as low as 4.0 J/cm2 were sufficient to induce morphological changes in the root surface. Additionally, for fluences reaching or exceeding 4.0 J/cm2, laser-induced inhibitory effects on root surface demineralization were observed. It was concluded that laser energy density in the range of 4.0 to 6.0 J/cm2 could be applied to a dental root to reduce demineralization of this surface without compromising pulp vitality.

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