Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites
Miriam H. Kossuga, Stelamar Romminger, Camila Xavier, Marília C. Milanetto, Milene Z. do Valle, Eli F. Pimenta, Raquel P. Morais, Erica de Carvalho, Carolina M. Mizuno, Luís Fernando C. Coradello, Vinícius de M. Barroso, Bruna Vacondio, Darci C. D. Javaroti, Mirna H. R. Seleghim, Bruno C....
Miriam H. Kossuga, Stelamar Romminger, Camila Xavier, Marília C. Milanetto, Milene Z. do Valle, Eli F. Pimenta, Raquel P. Morais, Erica de Carvalho, Carolina M. Mizuno, Luís Fernando C. Coradello, Vinícius de M. Barroso, Bruna Vacondio, Darci C. D. Javaroti, Mirna H. R. Seleghim, Bruno C. Cavalcanti, Claudia Pessoa, Manoel O. Moraes, Bruna A. Lima, Reginaldo Gonçalves, Rafaella C. Bonugli-Santos, Lara D. Sette, Roberto G. S. Berlinck
ARTIGO
Inglês
In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation...
In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K.
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
Aberto
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites
Miriam H. Kossuga, Stelamar Romminger, Camila Xavier, Marília C. Milanetto, Milene Z. do Valle, Eli F. Pimenta, Raquel P. Morais, Erica de Carvalho, Carolina M. Mizuno, Luís Fernando C. Coradello, Vinícius de M. Barroso, Bruna Vacondio, Darci C. D. Javaroti, Mirna H. R. Seleghim, Bruno C....
Miriam H. Kossuga, Stelamar Romminger, Camila Xavier, Marília C. Milanetto, Milene Z. do Valle, Eli F. Pimenta, Raquel P. Morais, Erica de Carvalho, Carolina M. Mizuno, Luís Fernando C. Coradello, Vinícius de M. Barroso, Bruna Vacondio, Darci C. D. Javaroti, Mirna H. R. Seleghim, Bruno C. Cavalcanti, Claudia Pessoa, Manoel O. Moraes, Bruna A. Lima, Reginaldo Gonçalves, Rafaella C. Bonugli-Santos, Lara D. Sette, Roberto G. S. Berlinck
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites
Miriam H. Kossuga, Stelamar Romminger, Camila Xavier, Marília C. Milanetto, Milene Z. do Valle, Eli F. Pimenta, Raquel P. Morais, Erica de Carvalho, Carolina M. Mizuno, Luís Fernando C. Coradello, Vinícius de M. Barroso, Bruna Vacondio, Darci C. D. Javaroti, Mirna H. R. Seleghim, Bruno C....
Miriam H. Kossuga, Stelamar Romminger, Camila Xavier, Marília C. Milanetto, Milene Z. do Valle, Eli F. Pimenta, Raquel P. Morais, Erica de Carvalho, Carolina M. Mizuno, Luís Fernando C. Coradello, Vinícius de M. Barroso, Bruna Vacondio, Darci C. D. Javaroti, Mirna H. R. Seleghim, Bruno C. Cavalcanti, Claudia Pessoa, Manoel O. Moraes, Bruna A. Lima, Reginaldo Gonçalves, Rafaella C. Bonugli-Santos, Lara D. Sette, Roberto G. S. Berlinck
Fontes
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Revista brasileira de farmacognosia Vol. 22, no. 2 (Mar./Apr., 2012), p. 257-267 |