Bone and skin tissue engineering based on cytotoxicity assays of PLLA fibrous mats synthesized via centrifugal spinning
Bárbara Etruri Ciocca, Nahieh Toscano Miranda, Guinea Brasil Camargo Cardoso, Ana Amélia Rodrigues, Ana Flávia Pattaro, André Luiz Jardini Munhoz, Rubens Maciel Filho
ARTIGO
Inglês
Poly (L-lactic acid) (PLLA) is a biocompatible polymer widely used in a range of applications and is already recognized in the medical field as a biomaterial. The main objective of the present work
was the production of polymeric mats to be applied in tissue engineering. PLLA mats were produced... Ver mais Poly (L-lactic acid) (PLLA) is a biocompatible polymer widely used in a range of applications and is already recognized in the medical field as a biomaterial. The main objective of the present work
was the production of polymeric mats to be applied in tissue engineering. PLLA mats were produced through the centrifugal spinning process. Scanning electron microscopy (SEM) analyzed the fibers diameter, disposition, and morphology. The fibers presented a uniform and constant diameter (16.24 µm), being arranged randomly. Thermogravimetric analysis (TGA) technique verified variation of
mass loss with temperature, in which the material degradation temperature started at 205 °C. Differential scanning calorimetry (DSC) determined phase transition temperatures. Fourier-transform infrared spectroscopy (FT-IR) evaluated PLLA fibrous membrane chemical groups. Cytotoxicity assays verified fibroblasts and osteoblasts viabilities by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide), AlamarBlue®, and Live/Dead® for 24, 48, and 72 h. This work results demonstrated that PLLA mats have great potential for tissue engineering applications in wound healing
and bone repairing, being a promising biomaterial with high biocompatibility Ver menos
was the production of polymeric mats to be applied in tissue engineering. PLLA mats were produced... Ver mais Poly (L-lactic acid) (PLLA) is a biocompatible polymer widely used in a range of applications and is already recognized in the medical field as a biomaterial. The main objective of the present work
was the production of polymeric mats to be applied in tissue engineering. PLLA mats were produced through the centrifugal spinning process. Scanning electron microscopy (SEM) analyzed the fibers diameter, disposition, and morphology. The fibers presented a uniform and constant diameter (16.24 µm), being arranged randomly. Thermogravimetric analysis (TGA) technique verified variation of
mass loss with temperature, in which the material degradation temperature started at 205 °C. Differential scanning calorimetry (DSC) determined phase transition temperatures. Fourier-transform infrared spectroscopy (FT-IR) evaluated PLLA fibrous membrane chemical groups. Cytotoxicity assays verified fibroblasts and osteoblasts viabilities by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide), AlamarBlue®, and Live/Dead® for 24, 48, and 72 h. This work results demonstrated that PLLA mats have great potential for tissue engineering applications in wound healing
and bone repairing, being a promising biomaterial with high biocompatibility Ver menos
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2008/57680-3
Fechado
Texto completo: http://www.euacademic.org/PastIssueList.aspx?artid=84
Bone and skin tissue engineering based on cytotoxicity assays of PLLA fibrous mats synthesized via centrifugal spinning
Bárbara Etruri Ciocca, Nahieh Toscano Miranda, Guinea Brasil Camargo Cardoso, Ana Amélia Rodrigues, Ana Flávia Pattaro, André Luiz Jardini Munhoz, Rubens Maciel Filho
Bone and skin tissue engineering based on cytotoxicity assays of PLLA fibrous mats synthesized via centrifugal spinning
Bárbara Etruri Ciocca, Nahieh Toscano Miranda, Guinea Brasil Camargo Cardoso, Ana Amélia Rodrigues, Ana Flávia Pattaro, André Luiz Jardini Munhoz, Rubens Maciel Filho
Fontes
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European academic research Vol. 7, no. 10 (Jan., 2020), p. 5044-5058 |