Separation of human Fab fragments on negative mode Ni(II)-TREN-agarose chromatography

Separation of human Fab fragments on negative mode Ni(II)-TREN-agarose chromatography

Luana Cristina Andrade da Silva, Marcel Mafei Serracchiani, Everson Alves Miranda, Sonia Maria Alves Bueno

ARTIGO

Inglês

We evaluated the feasibility of using immobilized metal-ion affinity chromatography (IMAC) with nickel ion complexed with Tris(2-aminoethyl)amine (TREN) immobilized on agarose gel for purification of human Fab fragments by negative chromatography. Efficient purification of Fab fragments from...

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

Fechado

Separation of human Fab fragments on negative mode Ni(II)-TREN-agarose chromatography

Luana Cristina Andrade da Silva, Marcel Mafei Serracchiani, Everson Alves Miranda, Sonia Maria Alves Bueno


										

Separation of human Fab fragments on negative mode Ni(II)-TREN-agarose chromatography

Luana Cristina Andrade da Silva, Marcel Mafei Serracchiani, Everson Alves Miranda, Sonia Maria Alves Bueno

    Fontes

    Process biochemistry

    Vol. 49, no. 4 (Apr., 2014), p. 715-723