Separation of human IgG fragments using copper, nickel, zinc, and cobalt chelated to CM-Asp-agarose by positive and negative chromatography

Separation of human IgG fragments using copper, nickel, zinc, and cobalt chelated to CM-Asp-agarose by positive and negative chromatography

Cecilia Alves Mourao, Gabriela Pannunzio Carmignotto, Sonia Maria Alves Bueno

ARTIGO

Inglês

This study evaluated the feasibility of using immobilized metal-ion affinity chromatography (IMAC) for separation of human Fab fragments using four different transition metal ions copper, nickel, zinc, and cobalt chelated to CM-Asp (carboxymethylaspartate) immobilized on the agarose gel. The Fab and...

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

Fechado

Separation of human IgG fragments using copper, nickel, zinc, and cobalt chelated to CM-Asp-agarose by positive and negative chromatography

Cecilia Alves Mourao, Gabriela Pannunzio Carmignotto, Sonia Maria Alves Bueno


										

Separation of human IgG fragments using copper, nickel, zinc, and cobalt chelated to CM-Asp-agarose by positive and negative chromatography

Cecilia Alves Mourao, Gabriela Pannunzio Carmignotto, Sonia Maria Alves Bueno

    Fontes

    Journal of chromatography B, analytical technologies in the biomedical and life sciences

    Vol. 1017 (Apr., 2016), p. 163-173